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Rivista di Pediatria, Neonatologia, Medicina dell’Adolescenza
e Neuropsichiatria Infantile
Indexed/Abstracted in: CAB, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,532
Minerva Pediatrica 2016 May 11
Standard and immunoproteasomes expression in paediatric tonsillectomy patients
Massimiliano BERGALLO 1, 2, Elisa LOIACONO 1, 2, Stefano GAMBARINO 1, 2, Luca VERGANO 1, 2, Paolo TAVORMINA 3 ✉
1 Department of Public Health and Pediatric Sciences, University of Turin, Medical School, Turin, Italy; 2 Laboratory of Citoimmunodiagnostics, University Hospital City of Science and Health of Turin, Regina Margherita Children's Hospital, Turin, Italy; 3 Unit of Otorhinolaryngology, Pediatric Hospital Regina Margherita S. Anna, Turin, Italy
BACKGROUND: Pro-inflammatory cytokines including TNFα and IFN-γ, play an important role during the recurrent tonsillitis illness and proteasome and immunoproteasome stimulation.
METHODS: mRNA expression analysis of proteasome and immunoproteasome subunits was performed by PBMC whole blood isolation from children tonsillectomy patients. Total RNA was extracted from PBMC and retro transcribed in cDNA., A Real Time PCR with Taqman probe was then performed.
RESULTS: PBMC of children with tonsillectomy showed, a significantly increased expression of proteasome constitutive subunit β2 encoding (1.52 ± 0.61 vs 0.88 ± 0.5, p <0.0001) compared to healthy controls (HC). The same results were observed for immunoproteasome inducible subunit LMP2 (2.55 ± 1.95 vs 0.73 ± 0.49, p <0.001), LMP7 (1.94 ± 1.18 vs 0.79 ± 0.41, p < 0.001), MECL1 (1.97 ±1.20 vs 0.79 ± 0.41, p < 0.001). No differences were observed for proteasome subunit β1. Conversely a significantly decreased expression of proteasome constitutive subunit β5 encoding mRNA (0.96 ±0.39 vs 1.28 ± 0.65, p = 0.0291) was observed.
CONCLUSIONS: In children with recurrent tonsillitis was observed an increased expression of β2, the corresponding iPS, β1i and β5i compared to healthy controls. Contrariwise β5 subunit mRNA expression was significantly decreased.