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Indexed/Abstracted in: CAB, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,532
Online ISSN 1827-1715
Massimiliano BERGALLO 1, 2, Elisa LOIACONO 1, 2, Stefano GAMBARINO 1, 2, Luca VERGANO 1, 2, Paolo TAVORMINA 3
1 Department of Public Health and Pediatric Sciences, University of Turin, Medical School, Turin, Italy; 2 Laboratory of Citoimmunodiagnostics, University Hospital City of Science and Health of Turin, Regina Margherita Children's Hospital, Turin, Italy; 3 Unit of Otorhinolaryngology, Pediatric Hospital Regina Margherita S. Anna, Turin, Italy
BACKGROUND: Pro-inflammatory cytokines including TNFα and IFN-γ, play an important role during the recurrent tonsillitis illness and proteasome and immunoproteasome stimulation.
METHODS: mRNA expression analysis of proteasome and immunoproteasome subunits was performed by PBMC whole blood isolation from children tonsillectomy patients. Total RNA was extracted from PBMC and retro transcribed in cDNA., A Real Time PCR with Taqman probe was then performed.
RESULTS: PBMC of children with tonsillectomy showed, a significantly increased expression of proteasome constitutive subunit β2 encoding (1.52 ± 0.61 vs 0.88 ± 0.5, p <0.0001) compared to healthy controls (HC). The same results were observed for immunoproteasome inducible subunit LMP2 (2.55 ± 1.95 vs 0.73 ± 0.49, p <0.001), LMP7 (1.94 ± 1.18 vs 0.79 ± 0.41, p < 0.001), MECL1 (1.97 ±1.20 vs 0.79 ± 0.41, p < 0.001). No differences were observed for proteasome subunit β1. Conversely a significantly decreased expression of proteasome constitutive subunit β5 encoding mRNA (0.96 ±0.39 vs 1.28 ± 0.65, p = 0.0291) was observed.
CONCLUSIONS: In children with recurrent tonsillitis was observed an increased expression of β2, the corresponding iPS, β1i and β5i compared to healthy controls. Contrariwise β5 subunit mRNA expression was significantly decreased.