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Indexed/Abstracted in: Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 1,236
Online ISSN 1827-1669
Shi J. 1, Liu J. 2, Liao L. 3, Guo Y. 2, Wang H. 2, Hu W. 4, Hu T. 5
1 Department of Medical Oncology Fourth Hospital of Hebei Medical University Shijiazhuang, China;
2 Key Laboratory of Animal Physiology Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences Hebei Normal University Shijiazhuang, Hebei, China;
3 Academy of Integrative Medicine Fujian University of Traditional Chinese Medicine, Fuzhou, China;
4 Department of Medical Oncology People hospital of Leling, Shandong, China;
5 Department of Medicine, Hebei Province Cangzhou City Integration of Chinese and Western Medicine Hospital East Cangzhou, China
AIM: Detection of novel tumor biomarker will aid in diagnosis of early-stage small cell lung cancer (SCLC). The purpose of this study was to identify novel tumor biomarker in serum from patients with SCLC using a proteomics-based approach.
METHODS: Sera were analyzed before the initiation of chemotherapy. Serum proteins of SCLC patients and healthy controls were collected and separated by 2-D fluorescence differential gel electrophoresis (2-D DIGE). Positive spots were analyzed by LC-MS/MS. Different expression of identified biomarker was verified by immunohistochemical method in wax specimen from 40 patients.
RESULTS: A total of 86 proteins were shown to be differentially abundant between the serum of SCLC patients and normal subjects by 2-D DIGE. Fifteen proteins were identified by LC-MS/MS. According to the bioinformatic analysis, these proteins are mainly involved in development and carcinogenesis. Some of them have been previously demonstrated to be important prognostic factors. Differential expression of 5 proteins between the normal tissue and cancerious tissue was confirmed by immunochemistry of SCLC patients.
CONCLUSION: We have identified different serum proteins between SCLC patients and healthy controls. These proteins may be potential serum biomarkers for early detection of SCLC and play a role in the development and metastasis of SCLC.