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Minerva Ginecologica 2016 Jul 19

lingua: Inglese

The evaluation of alteration of ELAM-1 expression in the endometriosis patients

Hoda BAHRAMI 1, 3, 4, Reza ROOZAFZOON 1, Zinat GHANBARI 2, Neda JEGARGOOSHE 3, Parvaneh JAFARI 5, Mohammad NAZARI 6, Saeed HEIDARI-KESHEL 1

1 Stem Cell Preparation Unit, Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran; 2 Departments of Gynecology and Obstetrics, Tehran University of Medical Sciences, Tehran, Iran; 3 Academy of Gengostar, Karaj, Iran; 4 Biotechnology Department, Science & Research Branch, Islamic Azad University, Damghan, Iran; 5 Microbiology Departments, Science Faculty, Science and Research Branch, Islamic Azad, University, Arak, Iran; 6 Department of Chemistry, Faculty of Science and Engineering, York University, Toronto, Ontario, Canada


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BACKGROUND: As a definition, Endometriosis is a commonly encountered gynecological disease; as endometrial glandular and stromal cells existing in the outside of the uterus. In spite of several studies on endometriosis, its molecular mechanism and pathogenesis nevertheless have been not completely illuminated. Role of ELAM-1 gene in mediating the adhesion of tumor cells to endothelial cells by binding to ELAM-1 ligands expressed by eosinophils, neutrophils, monocytes, natural killer cell effectors T-lymphocyte cell, and cancer (progenitor/stem) cells is well studied. Our hypothesis proposes that the alteration in ELAM-1 mRNA level is a significant point in successful transplantation deregulations of effective migration genes which may be related to endometrial proliferative disorders such as endometriosis.
METHODS: In this study, Real time-PCR, Western blotting and immunohistochemistry has been applied in order to determine the expression pattern of ELAM-1 gene in 22 patients. for the validation study, control group include 8 healthy wemon were recruited. Comparison of the prognostic accuracy of Elam-1 with other clinicopathological risk factors were analyzed by logistic regression.
RESULTS: The endometriosis tissues from women ages 33 ± 7 years (mean, [±SEM]) were assessed. The results showed that the ELAM-1 mRNA expression is significantly higher in patients group compare control group (p=0.0001). Moreover, in the samples of Stage III and IV, ELAM-1 gene has expressed more (p<0.001). ELAM-1 immunostaining expressed positive staining in the patients group. ELAM-1 protein expression level was correlated directly with American Fertility Society score and depicted no correlation with age.
CONCLUSIONS: According to our results, ELAM-1 upregulation may promote disorder in adhesion, and migration of the cells leading to the endometriosis disorder.

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Bahrami.hoda@gmail.com