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Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,246
Online ISSN 1827-160X
Hao L., Shi Z. D., Hu F. F., Zhang Z. G., Dong Y., Zhang J. J., Han C. H.
Department of Urology, Xuzhou Central Hospital, Xuzhou, Jiangsu, China
AIM: The aim of this paper was to study the mechanisms of gambogic acid on bladder cancer cell apoptosis and cell cycle arrest.
METHODS: Human bladder cancer BIU-87 cell lines were cultured in vitro. Different concentrations of gambogic acids were added to the logarithmic growth phase in the human bladder cancer BIU-87 cells for further cultivation. Caspase-3 protein expression in tumor tissue was detected by immunohistochemical S-P method. The gambogic acid-induced apoptosis in human bladder cancer cells and cell cycle changes were detected and analyzed using flow cytometry.
RESULTS: The expression of Caspase-3 was detected by immunohistochemistry. The results were as follows: control group, gambogic acid 1.0 μmol/L group, 2.0 μmol/L group, and 3.0 μmol/L group. Histoimmunochemical scoring (HIS) of the results were: 2.13±1.27, 4.28±1.86, 5.03±0.78, 6.47±1.31. The differences between each group and the control group were all statistically significant (P<0.05). Flow cytometry results showed that: the cells decreased gradually in G0/G1 phase of cell cycle and increased gradually in G2/M phase. No obvious change was observed in S phase cells.
CONCLUSION: Gambogic acid can promote bladder cancer cell apoptosis by increasing the expression of Caspase-3 protein and can arrest the cell cycle of bladder cancer cells, and inhibit the proliferation of bladder cancer cells.