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Rivista di Biologia Molecolare e Biotecnologie

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Minerva Biotecnologica 2014 December;26(3):301-7

lingua: Inglese

Peanut pod-an inexpensive substrate for β-galactosidase production by Bacillus sp. in solid-state fermentation: process evaluation and optimization by Taguchi design of experimental (DOE) methodology

Khayati G. 1, Anvari M. 2, Kazemi S. 3

1 Department of Chemical Engineering, Technical Faculty, Guilan University, Rasht, Iran;
2 Department of Microbiology, Faculty of Sciences, Islamic Azad University, Rasht Branch, Rasht, Iran;
3 Department of Microbiology, Sciences and Research Branch, Islamic Azad University, Rasht, Iran


AIM: The aim of present study was β-galactosidase production from indigenous and inexpensive wastes under ssf conditions using statistical experimental design
METHODS: Design of experimental (DOE) methodology using Taguchi orthogonal array (OA) was applied to evaluate the influence of five factors (peanut pod concentration, C/N ratio, incubation time, type of solid substrate and lactose concentration) on the β-galactosidase production by Bacillus licheniformis under solid-state fermentation.
RESULTS: The results showed that peanut pod concentration, incubation time and lactose (as inducer) were found to be the most effective factor for promoting enzyme production, followed by the C/N ratio. Peanut pod 70%w/w, C/N ratio 20, incubation time 72h, wheat straw as solid substrate and lactose concentration 1%w/w the best conditions determined by the Taguchi method.
CONCLUSION: Our finding showed that combination of peanut pod with wheat and rice straw could be used as suitable and low-cost substrate for β-galactosidase production. Also, the study shows that the Taguchi’s method is suitable to optimize the experiments for the production of β-galactosidase.

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