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Rivista di Biologia Molecolare e Biotecnologie


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Minerva Biotecnologica 2013 Dicembre;25(4):219-25

lingua: Inglese

Relationship between total carbon, total nitrogen and carbon to nitrogen ratio on growth, sporulation rate and δ-endotoxin synthesis of Bacillus thuringiensis

Mazmira M. M. M. 1, 2, Ramlah S. A. A. 1, Rosfarizan M. 2, Ling T. C. 3, Ariff A. B. 2

1 Biological Research Division, Malaysian Palm Oil Board Kajang, Selangor, Malaysia;
2 Department of Bioprocess Technology Faculty of Biotechnology and Biomolecular Sciences , Universiti Putra Malaysia, Selangor, Malaysia;
3 Institute of Biological Sciences , Faculty of Science Building, University of Malaya, Kuala Lumpur, Malaysia


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The relationship between intracellular and extracellular total carbon, total nitrogen and carbon to nitrogen ratio on Bacillus thuringiensis MPK13 growth, sporulation rate and d-endotoxin synthesis were carried out in shake flask using different types of carbon (glucose, galactose, sucrose, lactose and maltose) and nitrogen (yeast extract) sources. The highest initial intracellular TC (5.15 g/L) and intracellular TN (1.45 g/L) were recorded in medium containing glucose. On the other hand, the highest initial extracellular TC (34.54 g/L) and TN (7.61 g/L) were recorded in medium containing maltose and lactose, respectively. At 48 h of cultivation, the highest final intracellular TC (11.96 g/L) and intracellular TN (3.26 g/L) were also recorded in medium containing glucose. The highest final extracellular TC (27.85 g/L) and TN (7.27 g/L) were recorded in medium containing galactose which showed the lowest growth and sporulation. The presence of d-endotoxin was only detected at 48 h of cultivation using medium containing glucose. The result indicated that high initial intracellular TC and TN values during the cultivation anticipated in high cell growth, sporulation and d-endotoxin production and may be used for indirect measurement of cultivation performance for B. thuringiensis.

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arbarif@biotech.upm.edu.my