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Rivista di Biologia Molecolare e Biotecnologie

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Minerva Biotecnologica 2011 June-September;23(2-3):65-70


lingua: Inglese

Serotyping of Escherichia coli from healthy cattle and analyses of antimicrobial activities of Chinese herbal drugs on Escherichia coli strains with different serotypes in Hulunbeier, China

Simujide H. 1, Aorigele C. 1, Chun-Jie W. 2, Manda B.

1 College of Animal Science, Inner Mongolia Agricultural University, Hohhot, P. R. China; 2 College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, P. R. China


Aim. The aim of this paper was to provide a scientific evidence for means of spread and infection patterns of Escherichia coli (E. coli), serotype distribution and drug resistance ability of E. coli strains from apparently healthy dairy cows were examined.
Methods. Based on the results of isolation and serotyping tests, among 212 animals investigated, 101 (47.64 %) were proved to carry E. coli strains. Except 15 isolates not been serotyped, 86 of total 101 isolates from different cattle distributed among 40 kinds of serotypes, some of which were known to be pathogenic for humans and animals, i.e., O2, O8, O78 and O149.
Results and conclusion. According to the findings of antimicrobial study, all of the five Chinese herbal drugs (Rhizoma coptidis, Fructrs trichosanthis, Atractylodes chinensis, Scutellaria baicalensis georgi and Taraxaci mongolicum) used were active against E. coli with different serotypes at different extent. Atractylodes chinensis had the best antimicrobial activity, of which the minimum inhibitory concentrations (MIC) ranged from 0.031 g·mL-1 to 0.125 g·mL-1, the minimal value of the minimum bactericidal concentration (MBC) was 0.062 g·mL-1. Rhizoma coptidis was the most inactive one against E. coli exhibiting MIC from 0.250 g·mL-1 to 0.500 g·mL-1 and no MBC values in concentrations range. The antimicrobial effects of Fructrs trichosanthis, Scutellaria baicalensis georgi and Taraxaci mongolicum were between the former two. Moreover, a change in antimicrobial activity of each drug was also found when it was tested against E. coli with different kinds of serotypes.

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