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Minerva Biotecnologica 2011 June-September;23(2-3):53-7

lingua: Inglese

Occurrence of Aflatoxin M1 in Raw and pasteurized milk in Esfahan province of Iran

Daraei Garmakhany A. 1, Zighamian H. 2, Sarhangpour R. 2, Rasti M. 3, Aghajani N. 1

1 Yang Researchers Club, Georgan Branch, Arad, University, Gorgan, Iran;
2 Members of Food Quality Control Laboratory of EbneSina No. 22, Esfahan, Iran;
3 Member of Scientific Board of Esfahan Natural Resources and Agricultural Research Esfahan, Iran


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Aflatoxin M1 (AFM1) in milk and milk products is considered to pose certain hygienic risks for human health. During September to November 2009, a total of 74 samples of raw and pasteurized milk commercialized in the Esfahan province of Iran were analyzed for the presence of aflatoxin M1 (AFM1) by using an immunoaffinity column for clean up and HPLC with fluorescence detector for determination. Sixty-three samples (85.14%) of total (both raw and pasteurized milk) samples were found to be contaminated with AFM1, and 8 samples (10.81%) exceeded the legal level of AFM1 in milk according to the Iranian Food Codex and Codex Alimentarius limit (0.05 µg/kg). AFM1 was detectectd in 83.78% (31) and 86.49% (32) of raw and pasteurized milk samples but only 5.41% (2) and 16.22% (6) of raw and pasteurized milk samples respectively exceeded the legal level of AFM1 in milk (0.05 µg/kg). The maximum amount of AFM1 in raw and pasteurized milk samples were 0.165 and 0.108 (0.05 µg/kg) while the minimum amount of AFM1 were 0 and 0.002 (0.05 µg/kg) respectively. Due to presence of AFM1 in dairy products and its serious risks for public health, these products must be controlled periodically for AFM1 contamination.

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