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Rivista di Biologia Molecolare e Biotecnologie
Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
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Minerva Biotecnologica 2010 Giugno;22(2):39-45
Cultivation and quality assessment of tissue cultures
in Panax ginseng C. A. Meyer
Huang T. 1, Gao W. 1,2, Wang J. 1,3, Zhao Y. 3, Huang L. 4, Liu C. 5
1 School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China
2 Institute of Biological Engineering of Traditional Chinese Medicine, Tianjin University of Science and Technology, Tianjin,China
3 The College of Chinese Medicinal Materials, Jilin Agricultural University, Jilin, China
4 Pharmaceutical Research Institute of China, Academy of Traditional Chinese Medicine, Beijin, China
5 School of Chemical Engineering, Tianjin University, Tianjin, China
Aim. To assess the qualities of ginseng callus culture, adventitious root culture, as well as solid-cultured and liquid-cultured hairy root culture, and then try to find the best one for producing ginseng active components.
Methods. Biomass growth, ginsenoside and polysaccharide production in calli, adventitious roots and hairy roots of Panax ginseng C. A. Meyer were observed, and part of data were analyzed using cluster analysis to estimate more scientifically.
Results. The adventitious root culture grew very fast although it was still a little slower than liquid-cultured hairy root culture. The content of ginsenosides in adventitious root culture was much higher than that in other tissue cultures and was even higher than that in its explant resource, namely taproots of field cultivation ginseng. In addition, there seemed to be a much lower content of polysaccharides and a low ratio of Rb-group to Rg-group in ginseng tissue cultures comparing to field-cultivation ginseng roots. Based on the cluster analysis of ginsenoside content data, a new method seemed to be found to distinguish native ginseng and different in vitro cultures from each other.
Conclusion. Adventitious root culture had a greater capability of ginsenoside production comparing to callus and hairy root culture, and its quality was very close to that of native ginseng.