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Rivista di Biologia Molecolare e Biotecnologie
Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
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Minerva Biotecnologica 2003 September;15(3):185-9
Xenotransplantation of alginate-chitosan-polyethyleneglycol microencapsulated rat islets in the treatment of mice diabetes mellitus. Preliminary data and future perspective
Lou W., Qin X., Wu Z.
Department of General Surgery, Zhong Shan Hospital, FuDan University, Shangai, China
Aim. To compare the results of xenotransplantation of Alginate-polylysine-alginate microencapsulated rat islets (APA microencapsulated group) and alginate-chitosan-polyethyleneglycol microencapsulated rat islets (ACP microencapsulated group) in the treatment of mice diabetes mellitus.
Methods. Streptozotosin 220 mg/kg intra-abdominal injection prepared the C57BL/6 mice diabetic model. Three groups were assigned: naked rat islet group; APA microencapsulated islets transplantation group and ACP microencapsulated islets transplantation group; 200 of each kind of islets were transplanted into the abdominal cavities of diabetic mice in the respective groups. The levels of blood glucose were recorded before and after the transplantation.
Results. One day after transplantation, the level of blood glucose of all groups was returned to normal. On the 2nd day, the level of blood glucose of the naked islet group was back to the pretransplantation level, while that of the APA microencapsulated group and the ACP microencapsulated group were kept in the normal range for 57±14.61 and 41.67±16.73 days after transplantation respectively. No difference was observed between these 2 groups.
Conclusion. The ACP microcapsule could provide immune isolation to xeno-islets as the APA microcapsule does; transplantation of APA microencapsulated rat islets could reverse the diabetic state of mice.