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ORIGINAL ARTICLES  TRENDS IN MOLECULAR DIAGNOSIS AND THERAPY OF β-THALASSEMIA AND SICKLE CELL ANEMIA


Minerva Biotecnologica 2003 June;15(2):93-7

Copyright © 2003 EDIZIONI MINERVA MEDICA

lingua: Inglese

Recent advanc­es in molec­u­lar diag­no­sis using sur­face plas­mon res­o­nance and bio­sen­sor tech­nol­o­gy for detec­tion of β-thalassemia mutations

Breveglieri G. 1,2, Gardenghi S. 2, Gambari R. 1, 2, Feriotto G. 1

1 Biotechnology Center, University of Ferrara, Ferrara, Italy;
2 Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy


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In the ­present ­review we ­describe a new meth­o­dol­o­gy employ­ing sur­face plas­mon res­o­nance (SPR) and bio­sen­sor tech­nol­o­gy to ­detect ­point muta­tions, includ­ing ­those caus­ing a ­severe ­form of β°­IVSI-1 tha­las­se­mia. The ­data pre­sent­ed ­allow to con­clude ­that bio­spe­cif­ic inter­ac­tion anal­y­sis (BIA) is a ­fast and auto­mat­able ­approach for detect­ing muta­tions of the β-glo­bin ­gene by the ­real-­time mon­i­tor­ing of hybrid­iza­tion ­between oli­go­nu­cleo­tide (ODN) ­probes and tar­get bio­tin­y­lat­ed poly­me­rase-­chain reac­tion (PCR) prod­ucts gen­er­at­ed ­from genom­ic DNA of nor­mal, het­er­o­zy­gous sub­jects and homo­zy­gous β°-tha­las­se­mia ­patients and immob­il­ized on strep­tav­i­din coat­ed sen­sor ­chips. During the asso­ci­a­tion ­phase, dis­crim­i­na­tion ­between mis­matched and ­full ­matched ODN/PCR prod­uct ­hybrids is read­i­ly and repro­du­cibly ­observed.

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