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Rivista di Biologia Molecolare e Biotecnologie
Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
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MICROARRAY MEETING 2002: NEW DEVELOPMENTS IN MUTATION DETECTION AND GENE EXPRESSION
Segrate, MI (Italy), April 12, 2002
Minerva Biotecnologica 2002 December;14(3-4):273-9
Gene expression profiling in skeletal muscle using a dedicated microarray
Campanaro S. 1, Romualdi C. 1, De Pittà C. 1, Fanin M. 2, Celegato B. 1, Pacchioni B. 1, Trevisan S. 1, Laveder P. 1, Toppo S. 1, Cagnin S. 1, Valle G. 1, Angelini C. 2, Lanfranchi G. 1
1 CRIBI Biotechnology Center, University of Padua, Padua, Italy;
2 Department of Psychiatric and Neurologic Sciences, University of Padua, Padua, Italy
Background. Microarrays can measure simultaneously the expression of thousands of genes and identify subtle changes in expression between different biological states. In our lab a novel two-step subtractive strategy was settled in order to enrich for genes expressed at low levels in skeletal muscle.
Methods. Using this cDNA collection we have developed a dedicated muscle-specific microarray composed of 2,700 sequences corresponding to the 400-500 most 3’-terminal region of muscle transcripts.
Results. The expression profile of limb-girdle dystrophy type 2B was analysed with our array.
Conclusions. We found approximately 3% differentially expressed genes, mostly involved in muscle contraction and immune response.