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  MICROARRAY MEETING 2002: NEW DEVELOPMENTS IN MUTATION DETECTION AND GENE EXPRESSION
Segrate, MI (Italy), April 12, 2002
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Minerva Biotecnologica 2002 December;14(3-4):273-9

Copyright © 2003 EDIZIONI MINERVA MEDICA

lingua: Inglese

Gene expression profiling in skeletal muscle using a dedicated microarray

Campanaro S. 1, Romualdi C. 1, De Pittà C. 1, Fanin M. 2, Celegato B. 1, Pacchioni B. 1, Trevisan S. 1, Laveder P. 1, Toppo S. 1, Cagnin S. 1, Valle G. 1, Angelini C. 2, Lanfranchi G. 1

1 CRIBI Biotechnology Center, University of Padua, Padua, Italy; 2 Department of Psychiatric and Neurologic Sciences, University of Padua, Padua, Italy


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Background. Microarrays can meas­ure simul­ta­ne­ous­ly the expres­sion of thou­sands of ­genes and iden­ti­fy sub­tle chang­es in expres­sion ­between dif­fer­ent bio­log­i­cal ­states. In our lab a nov­el two-­step sub­trac­tive strat­e­gy was set­tled in ­order to ­enrich for ­genes ­expressed at low lev­els in skel­e­tal mus­cle.
Methods. Using ­this cDNA col­lec­tion we ­have devel­oped a ded­i­cat­ed mus­cle-spe­cif­ic micro­ar­ray com­posed of 2,700 sequenc­es cor­re­spond­ing to the 400-500 ­most 3’-ter­mi­nal ­region of mus­cle tran­scripts.
Results. The expres­sion pro­file of ­limb-gir­dle dys­tro­phy ­type 2B was ana­lysed ­with our ­array.
Conclusions. We ­found approx­i­mate­ly 3% dif­fe­ren­tial­ly ­expressed ­genes, most­ly ­involved in mus­cle con­trac­tion and ­immune ­response.

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