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Minerva Biotecnologica 2002 December;14(3-4):259-63

Copyright © 2003 EDIZIONI MINERVA MEDICA

lingua: Inglese

Investigation of a subset of human polymorphisms by ligase detection reaction and universal array

Consolandi C. 1, Bordoni R. 2, Castiglioni B. 3, Frosini A. 1, Mezzelani A. 2, Rizzi E. 2, Battaglia C. 1, Pera C. 4, Ferrara G. B. 5, Zatta P. 2, Zambenedetti P. 6, Rossi Bernardi L. 1, De Bellis G. 2

1 Department of Biomedical Sciences and Technologies, University of Milan, Milan, Italy; 2 Institute of Biomedical Technologies, National Council for Researches, Milan, Italy; 3 Institute of Agriculture Biology and Biotechnologies; 4 Advanced Biotechnology Center, National Cancer Institute, Genoa, Italy; 5 Department of Oncology, Biology and Genetics, University of Genoa, Genoa, Italy; 6 Encephalon Bank, General Hospital, Dolo-Venice, Italy


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We present our results in the identification of human polymorphisms within the 2nd exon of the HLA-A region, in the exon 4 of apolipoprotein E and in the exon 15 of the transferrin gene. Allele specific detection was performed by PCR followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. This work confirmed the results previously obtained by sequence or enzymatic analysis thus demonstrating the feasibility of genotyping studies by LDR using a universal DNA array.

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