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  MICROARRAY MEETING 2002: NEW DEVELOPMENTS IN MUTATION DETECTION AND GENE EXPRESSION
Segrate, MI (Italy), April 12, 2002
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Minerva Biotecnologica 2002 December;14(3-4):253-7

Copyright © 2003 EDIZIONI MINERVA MEDICA

lingua: Inglese

Application of an universal DNA microarray to cyanobacterial diversity assessment

Castiglioni B. 1, Rizzi E. 2, Frosini A. 3, Mugnai M. A. 4, Ventura S. 4, Sivonen K. 5, Rajaniemi P. 5, Rantala A. 5, Wilmotte A. 6, Boutte C. 6, Consolandi C. 3, Bordoni R. 2, Mezzelani A. 2, Busti E. 3, Rossi Bernardi L. 3, Battaglia C. 3, De Bellis G. 2

1 Institute of Agricultural Biology and Biotechnology; 2 Institute of Biomedical Technology, National Council for Researches, Milan, Italy; 3 Department of Biomedical Sciences and Technology, University of Milan, Italy; 4 Institute for the Study of Echosystems, National Council for Researches, Florence, Italy; 5 University of Helsinki, Helsinki, Finland; 6 University of Liege, Liege, Belgium


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Our tech­no­log­i­cal ­goal was to ­test the new tech­nol­o­gy of DNA micro­ar­rays in the ­field of bio­di­ver­sity assess­ments. The effi­cien­cy of DNA micro­ar­rays was com­pared ­against the “clas­si­cal” meth­ods for meas­ur­ing and mon­i­tor­ing the bio­di­ver­sity. We use cya­no­bac­te­ria ­from fresh­wa­ter ­lakes as mod­el organ­isms belong­ing to a ­well ­defined phy­lum of ­much eco­log­i­cal rel­e­vance. The com­bi­na­tion of the infor­ma­tion ­about cya­no­bac­te­ri­al bio­di­ver­sity togeth­er ­with the ­data describ­ing the ­water qual­ity of the ­lakes may ­reveal use­ful infor­ma­tion to ­design man­age­ment ­actions to con­trol the unwant­ed ­mass devel­op­ments of cya­no­bac­te­ria. ­Such ­blooms pre­vent the use of ­water for drink­ing and for rec­re­a­tion and can be tox­ic to ani­mals and ­humans. The pos­sibil­ity to auto­mate the micro­ar­ray tech­nol­o­gy in ­water qual­ity mon­i­tor­ing ­will be an inter­est­ing alter­na­tive to ­time-con­sum­ing micro­scop­ic enu­mer­a­tions.

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