Home > Riviste > Minerva Biotecnologica > Fascicoli precedenti > Minerva Biotecnologica 2002 December;14(3-4) > Minerva Biotecnologica 2002 December;14(3-4):247-52

ULTIMO FASCICOLO
 

ARTICLE TOOLS

Estratti

MINERVA BIOTECNOLOGICA

Rivista di Biologia Molecolare e Biotecnologie


Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,25


eTOC

 

  MICROARRAY MEETING 2002: NEW DEVELOPMENTS IN MUTATION DETECTION AND GENE EXPRESSION
Segrate, MI (Italy), April 12, 2002
FREEfree


Minerva Biotecnologica 2002 December;14(3-4):247-52

Copyright © 2003 EDIZIONI MINERVA MEDICA

lingua: Inglese

Ligase detection reaction (LDR) and universal array (Zip Code): application to DNA genotyping

De Bellis G. 1, Castiglioni B. 2, Bordoni R. 1, Mezzelani A. 1, Rizzi E. 1, Frosini A. 3, Busti E. 3, Consolandi C. 3, Rossi Bernardi L. 3, Battaglia C. 3

1 Institute of Biomedical Technologies, National Council of Researches, Milan, Italy; 2 Institute of Agricultural Biology and Biotechnology, National Council for Researches, Milan, Italy; 3 Department of Biochemical Sciences and Technologies, CISI, University of Milan, Milan, Italy


FULL TEXT  


The Uni­ver­sal DNA micro­ar­ray ­approach has ­been pro­posed for set­ting up sev­er­al dif­fer­ent DNA anal­y­sis. ­Such an ­approach is ­based on an ­arrayed set of oli­go­nu­cleo­tides (the zip­code sequenc­es) hav­ing com­mon ther­mo­di­nam­ic beha­vi­our but ­being unre­lat­ed to any spe­cif­ic DNA ­sequence ­under ­study. ­This pro­ce­dure, ­that can be com­bined to liga­tion, exten­sion or min­i­se­quenc­ing, ­requires the ­design of a set of ­probes spe­cif­ic for ­each tar­get ­sequence (muta­tions, poly­mor­phism, etc.) append­ed ­with com­ple­men­tary Zip­Code (cZip­Code) sequenc­es. ­These ­probes are react­ed ­against a prop­er tar­get in solu­tion in a mul­ti­plexed for­mat ­using the ­above men­tioned enzy­mat­ic reac­tions. The result­ing prod­ucts, bear­ing ­their own cZip­Code are ­addressed to the loca­tion on the micro­ar­ray ­where the cor­re­spond­ing Zip­Code ­sequence has ­been spot­ted. ­Such an ­array is there­fore “Uni­ver­sal” ­being unre­lat­ed to a spe­cif­ic molec­u­lar anal­y­sis. ­Here we ­present the set up and opti­mi­za­tion of ­this pro­ce­dure and its appli­ca­tion to bac­te­ri­al dis­crim­i­na­tion. ­Four pres­en­ta­tions fol­low dem­on­strat­ing the flex­ibil­ity of the Uni­ver­sal ­array for­mat in detect­ing patho­gens and ­GMOs, mon­i­tor­ing bac­te­ri­al diver­sity and typ­ing ­SNPs.

inizio pagina

Publication History

Per citare questo articolo

Corresponding author e-mail