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ULTIMO FASCICOLOMINERVA BIOTECNOLOGICA

Rivista di Biologia Molecolare e Biotecnologie

Indexed/Abstracted in: EMBASE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,246

Periodicità: Trimestrale

ISSN 1120-4826

Online ISSN 1827-160X

 

Minerva Biotecnologica 2002 Dicembre;14(3-4):231-6

MICROARRAY MEETING 2002: NEW DEVELOPMENTS IN MUTATION DETECTION AND GENE EXPRESSION
Segrate, MI (Italy), April 12, 2002 

A ­review of strat­e­gies ­based on prim­er exten­sion (PEX) tech­nol­o­gy

Gemiani F. 1, Landi S. 1, 2, Canzian F. 1

1 Genome Anal­y­sis ­Group, Inter­na­tion­al Agen­cy for ­Research on Can­cer, Lyon, ­France;
2 Dipartimento di Scienze dell’Uomo e dell’Ambiente, Uni­ver­sity of ­Pisa, ­Pisa, Ita­ly

In prim­er exten­sion (PEX) reac­tion, a DNA poly­me­rase is ­used to ­extend spe­cif­i­cal­ly a prim­er ­that ­anneals imme­di­ate­ly adja­cent to the var­i­ant nucle­o­tide. The reac­tion ­allows high­ly spe­cif­ic detec­tion of ­point muta­tions and sin­gle nucle­o­tide poly­mor­phisms (­SNPs). ­Because all ­SNPs can be ana­lyzed ­with ­high spec­i­fic­ity at the ­same reac­tion con­di­tions, PEX is a prom­is­ing reac­tion prin­ci­ple for mul­ti­plex ­high-through­put gen­o­typ­ing ­assays. ­This ­review dis­cuss­es the pos­sible appli­ca­tions of PEX and dif­fer­ent approach­es of prim­er exten­sion reac­tions.

lingua: Inglese


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