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Rivista di Biologia Molecolare e Biotecnologie

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Minerva Biotecnologica 2000 December;12(4):271-8

lingua: Inglese

The ­effect of dif­fer­ent in ­vitro solu­tions in the dis­so­lu­tion of cal­cium pec­ti­nate ­beads intend­ed for the deliv­ery of ­live ­cells to the ­human ­large intes­tine

Chan W. A., Boswell C. D., Zhang Z.

School of Chemical Engineering, University of Birmingham, Edgbaston, B15 2TT, United Kingdom


Hydrogel ­formed by a low methox­yl pec­tin and cal­cium ion has poten­tial appli­ca­tion for tar­get­ed deliv­ery of bio­log­i­cal mate­ri­als to the ­human ­large intes­tine. Pectin ­does not dis­solve in the ­upper ­human gas­troin­tes­ti­nal (GI) ­tract but can be degrad­ed by pec­tin­o­lyt­ic ­enzymes ­present in the ­human ­large intes­tine. However, the com­po­si­tion of dif­fer­ent ­types of in-­vitro dis­so­lu­tion ­fluids ­designed to mim­ic ­human ­small intes­tine and ­large intes­tine ­fluids, can ­affect the dis­so­lu­tion pro­file of cal­cium pec­ti­nate (CaP) ­beads. The aim of ­this ­study was to com­pare the ­release pro­files of a bio­log­i­cal mate­ri­al in ­some com­mon­ly ­used phos­phate buf­fer solu­tions ­with ­release pro­files in phys­io­log­i­cal ­salts solu­tions, ­using the BP 98 pad­dle dis­so­lu­tion meth­od. It was ­found ­that the ­release pro­files for ­these two ­types of in-­vitro solu­tions ­were com­par­able with­out the pre-treat­ment ­with an ­acid solu­tion, but ­were mark­ed­ly dif­fer­ent fol­low­ing the ­acid treat­ment. In addi­tion, it was ­found ­that the pres­ence of cal­cium ion in the solu­tion affect­ed the activ­ity of the pec­tin­o­lyt­ic ­enzyme.

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