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Rivista di Angiologia
Official Journal of the , the International Union of Phlebology and the
Indexed/Abstracted in: BIOSIS Previews, Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
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International Angiology 2006 Giugno;25(2):175-8
Presence of prostacyclin receptor in arteriosclerotic human femoral artery
Muto A. 1, Kondo Y. 1, Yamamoto Y. 2, Yamada H. 2, Washimi O. 2, Miyauchi Y. 3, Kudo F. 4, Dardik A. 4, Nishibe T. 1
1 Department of Surgery, Division of Cardiovascular Surgery, Fujita Health University, Toyoake, Japan
2 Department of Orthopedic Surgery, Fujita Health University, Toyoake, Japan
3 Department of Bio Research, Kamakura Techno Science, Inc., Kamakura, Japan
4 Department of Vascular Surgery, Yale University, New Heaven, CT, USA
Aim. Prostacyclin, which is mainly synthesized by vascular endothelial cells, exerts antiplatelet and smooth-muscle-relaxant effects, thereby maintaining cardiovascular homeostasis. Prostacyclin analogues have been clinically proven to improve ischemic symptoms and prevent the occurrence of vascular events in the lower extremities of patients with arteriosclerosis obliterans. We examined the presence of prostacyclin receptor (IP receptor) in an arteriosclerotic human femoral artery.
Methods. Specimens of the femoral artery were obtained at the time of limb amputation from an 83-year-old woman. Atherosclerotic lesions and associated changes such as calcification were evident. The specimens were stained with hematoxylin and eosin, and processed for immunohistochemistry.
Results. A monolayer of cells was observed on the luminal side of the femoral artery. Single immunohistochemistry showed the presence of the IP receptors on cells of the luminal side of the femoral artery. Triple-immunofluorescence staining revealed colocalization of IP-receptor-positive cells and cells positive for von Willebrand factor, a marker of vascular endothelial cells.
Conclusion. We investigated the presence of the IP receptor in the human femoral artery immunohistochemically, and demonstrated their strong expression in endothelial cells. This finding suggests that prostacyclin or prostacyclin analogues may act on their receptors on endothelial cells in patients with arteriosclerosis obliterans.