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Official Journal of the , the International Union of Phlebology and the
Indexed/Abstracted in: BIOSIS Previews, Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,899
Online ISSN 1827-1839
Ariyoshi H., Aono Y., Tsuji Y., Ueda A., Tokunaga M., Uchide K., Sakon M., Monden M.
Department of Surgery II, Osaka University Medical School, Osaka, Japan
Background. Although adherence and aggregation of platelets on an active surface such as exposed subendothelial matrix or foreign surfaces is integral to the occlusion of blood vessels, its mode of action is not fully understood.
Methods. The role of cytoplasmic ionized Ca2+ concentration ([Ca2+]i) in platelet activation induced by contact with a glass surface under shear-stress was studied by employing confocal laser scanning microscopy (CLSM) in conjunction with a parallel plate flow chamber. Changes in [Ca2+]i and morphology of aggregating platelets on glass surface was simultaneously examined.
Results. Under static condition, contact with glass caused platelet adhesion to the surface, which was associated with [Ca2+]i rise and morphological change; however, platelets did not develop a large aggregation on the surface. Under lower shear-stress, the number of the single platelets adsorbed on the surface was less than that under static condition. Although shear-stress increased the number of single platelets involved and enhanced morphological change in aggregating platelets in a shear-stress related manner, the peak [Ca2+]i value in individual platelets were not increased.
Conclusions. These observations may suggest the crucial roles of shear-stress in platelet aggregate formation at the site of arterial stenosis. Shear-stress might enhances platelet aggregate growth not through the enhancement of [Ca2+]i rise.