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Rivista di Angiologia

Official Journal of the International Union of Angiology, the International Union of Phlebology and the Central European Vascular Forum
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International Angiology 2002 March;21(1):58-62

lingua: Inglese

Accentuated oxidative stress following reperfusion injury in diabetic rats

Edrees W. K., Young I. S. *, Lau L. L., Rowlands B. J. **, Refsum S. E., Soong C. V.

Department of Surgery,
* Department of Clinical Biochemistry, The Queen’s University of Belfast, Belfast, Northern Ireland
** Department of Surgery, Queen’s Medical Centre, Nottingham, England, UK.


Background. This study inves­ti­gates wheth­er dia­betes mel­lit­us accen­tu­ates hind limb ische­mia-reper­fu­sion inju­ry.
Methods. Male Wistar rats ren­dered dia­bet­ic (n=40) fol­low­ing injec­tion of strep­to­zot­o­cin were com­pared to non-dia­bet­ic con­trol rats (n=30). Each group was divid­ed into sham, 4 hrs of hind limb ische­mia, 4 hrs of ische­mia fol­lowed by 10, 30 or 60 min of reper­fu­sion. Plasma con­cen­tra­tions of an end-prod­uct of lipid per­ox­i­da­tion [mal­on­di­al­de­hyde (MDA)] and anti­ox­i­dants (vita­mins A and E) were meas­ured togeth­er with the rest­ing mem­brane poten­tial (RMP) of the gas­troc­ne­mi­us mus­cle.
Results. Following reper­fu­sion, the dia­bet­ic group ­showed great­er and more per­sis­tent ele­va­tion of MDA and great­er reduc­tion of anti­ox­i­dants. This was asso­ciat­ed with reduc­tion in the RMP only in the dia­bet­ic group. There was sig­nif­i­cant cor­re­la­tion ­between MDA level and the RMP in both ­groups of ani­mals.
Conclusions. These ­results indi­cate that oxi­da­tive ­stress fol­low­ing reper­fu­sion inju­ry is great­er in the pres­ence of dia­betes mel­lit­us. This may lead to a ­decrease in the RMP and ­increase in the vas­cu­lar perme­abil­ity, which may be asso­ciat­ed with more com­pli­ca­tions.

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