Home > Riviste > International Angiology > Fascicoli precedenti > International Angiology 2000 June;19(2) > International Angiology 2000 June;19(2):166-70





Rivista di Angiologia

Official Journal of the International Union of Angiology, the International Union of Phlebology and the Central European Vascular Forum
Indexed/Abstracted in: BIOSIS Previews, Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 0,899




International Angiology 2000 June;19(2):166-70


lingua: Inglese

Pathological changes of hepatic artery and portal vein, after allyl-alcohol and carbon tetrachloride administration. An experimental study

Papalambros E., Felekouras E., Tsamandas A. *, Sigala F., Salakou S. *, Tepetes K. *, Filis K., Milonakis M., Kourelis T. *, Bastounis E.

From the First Depart­ment of Sur­gery and Depart­ment of His­tol­o­gy, Uni­ver­sity of Ath­ens Med­i­cal ­School Laiko Gen­er­al Hos­pi­tal,
* Depart­ment of ­Pathology, Uni­ver­sity of ­Patras Med­i­cal ­School, ­Greece

Full text temporaneamente non disponibile on-line. Contattaci  

Back­ground. All­lyl-­alchool (AA) and car­bon tet­ra­chlo­ride (CC14) are known to cause per­i­to­neal and per­i­cen­tral liver necro­sis, respec­tive­ly. This study inves­ti­gates path­o­log­ical chang­es of hepat­ic ­artery and por­tal vein after simul­ta­ne­ous admin­is­tra­tion of AA and CC14 in rats.
Meth­ods. The study com­prised 130 male ­Wistar rats divid­ed ran­dom­ly into 2 ­groups: I (n=10) sham and II (n=120) AA injec­tion (intra­per­it­o­neal­ly: 0.62 mmol/kg) and rhin­o­gas­tric admin­is­tra­tion of CC14 (0.66 ml/kg, 1:1 vol­ume dilu­tion in corn oil). After injec­tion was com­plet­ed, ani­mals of group II were ­assigned in 12 cat­e­go­ries and sac­ri­ficed 2, 4, 6, 12, 18, 24, 33, 48, 57, 81, and 153 hrs after. Tis­sue was ­obtained from the left ante­ri­or lobe and the hilum of the liver, and his­to­log­ical exam­ina­tion includ­ed H&E, sil­ver methen­a­mine and van Gies­son ­stains.
­Results. Liver sec­tions from group II (AA+CC14) dem­on­strat­ed per­i­por­tal togeth­er with per­i­cen­tral necro­sis; the peak was 57 hrs after injec­tion. In all 120 cases, H&E stain ­showed evi­dence of regen­er­a­tion orig­i­nat­ed from zone 2, extend­ing to zone 1 and occa­sion­al­ly to zone 3, and accom­plished main­ly by non-necrot­ic cell pro­life­ra­tion. Sec­tions from the liver hilum ­showed throm­bo­sis of the por­tal vein, where­as the hepat­ic ­artery and its branch­es devel­oped a varie­ty of chang­es. Initial­ly (2, 4 hrs), endo­the­lial hyper­tro­phy was ­observed which was fol­lowed by focal fib­ri­noid necro­sis of the arte­ri­al wall (6 hrs). Later on (9-153 hrs) the fol­low­ing find­ings were ­present: hyper­pla­sia and non-iso­met­ric cyto­plas­mic vac­uolisa­tion of media, dis­rup­tion of the elas­tic lam­i­na, aggre­ga­tion of foam cells and mac­ro­phag­es in inti­ma, media, and focal­ly in adven­ti­tia of hepat­ic ­artery; and lym­pho­cyt­ic inflam­ma­tion of inti­mal and per­i­ad­ven­ti­tial area. In 2 cases (153 hrs) hepat­ic ­artery throm­bo­sis was ­present.
Con­clu­sions. Addi­tion­ally to liver paren­chy­mal chang­es, simul­ta­ne­ous admin­is­tra­tion of allyl-alco­hol and car­bon tet­ra­chlo­ride in rats ­results to vas­cu­lar chang­es main­ly in the hepat­ic ­artery and its branch­es. Dur­ing liver paren­chy­mal regen­er­a­tion, the hepat­ic ­artery and its branch­es devel­op micro­scop­ic fea­tures that mor­pho­log­i­cal­ly resem­ble those of ath­ero­scler­o­sis. These chang­es may ­result in hepat­ic ­artery throm­bo­sis and or obstruc­tion.

inizio pagina

Publication History

Per citare questo articolo

Corresponding author e-mail