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Rivista di Angiologia

Official Journal of the International Union of Angiology, the International Union of Phlebology and the Central European Vascular Forum
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International Angiology 1999 December;18(4):331-4

lingua: Inglese

Effect of gly­cat­ed low den­sity lip­o­pro­tein on ­smooth mus­cle cell pro­life­ra­tion

Makita T., Tanaka A., Numano F.

From the Third Depart­ment of Inter­nal Med­i­cine, Tokyo Med­i­cal and Den­tal Uni­ver­sity, Tokyo, Japan


Back­ground. We inves­ti­gat­ed the ­effect of gly­cat­ed low den­sity lip­o­pro­tein (LDL) on smooth mus­cle cell pro­life­ra­tion.
Meth­ods. Blood was drawn from 6 ­healthy sub­jects after over­night fast­ing. ­Native LDL was ­obtained by sep­ar­at­ing LDL from the sam­ples with sequen­tial ultra­cen­trif­u­ga­tion. Gly­cat­ed LDL was pre­pared by gly­cat­ing the ­native LDL in vitro. ­Native and gly­cat­ed LDL were added to a medi­um con­tain­ing cul­tured por­cine cor­o­nary ­artery ­smooth mus­cle cells, and the ­change in cell pro­life­ra­tion was exam­ined after 24, 48, 72, and 96 hs. The cells were count­ed using a cell count­ing kit (Dojin Chem­i­cal Co., Ltd.).
­Results. There was no sig­nif­i­cant dif­fer­ence in the cell count ­between the con­trol group, in which only PBS was added, and the ­native LDL group. How­ev­er, cell pro­life­ra­tion was appre­ciably high­er in the gly­cat­ed LDL group than in the ­native LDL group. The mean total cell count at 24, 48, 72 and 96 hs was sig­nif­i­cant­ly high­er (p<0.01) in the gly­cat­ed LDL group (­median: 0.843; range: 0.576-1.060) than in the ­native LDL group (­median: 0.541; range: 0.282-0.683).
Con­clu­sions. These find­ings sug­gest that gly­cat­ed LDL induc­es sig­nif­i­cant­ly great­er accel­er­a­tion of ­smooth mus­cle cell pro­life­ra­tion than does ­native LDL. There­fore, the accel­er­a­tion of ­smooth mus­cle cell pro­life­ra­tion ­requires mod­ifi­ca­tion of LDL.

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