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INTERNATIONAL ANGIOLOGY

Rivista di Angiologia


Official Journal of the International Union of Angiology, the International Union of Phlebology and the Central European Vascular Forum
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International Angiology 1999 June;18(2):149-53

Copyright © 2000 EDIZIONI MINERVA MEDICA

lingua: Inglese

Importance of glycation in the acceleration of low density lipoprotein (LDL) uptake into macrophages in patients with diabetes mellitus

Makita T., Tanaka A., Nakano T., Nakajima K., Numano F.

From the Third Department of Internal Medicine, Tokyo Medical and Dental University, Tokyo, Japan * Japan Immunoresearch Laboratories, Takasaki City, Japan


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Back­ground. Two stud­ies were con­duct­ed to deter­mine the impor­tance of gly­ca­tion in the accel­er­a­tion of low den­sity lip­o­pro­tein (LDL) ­uptake by mac­ro­phag­es in ­patients with dia­betes mel­lit­us.
Meth­ods. ­Healthy indi­vid­u­als (n=6) and non-insu­lin­de­pen­dent dia­bet­ic ­patients (n=6) were stud­ied. LDL was sep­ar­at­ed by sequen­tial ultra­cen­trif­u­ga­tion. Mac­ro­phag­es were col­lect­ed from the abdom­i­nal cav­ity of ICR-mice and incu­bat­ed in a medi­um con­tain­ing [14C] ­oleate. In Study 1, LDL from dia­bet­ic ­patients (DM-LDL) and con­trol LDL were then incu­bat­ed with this medi­um. The ­uptake of DM-LDL by mac­ro­phag­es was com­pared with that of con­trol LDL by meas­ur­ing the [14c] con­tent of the syn­the­sized cho­les­te­ryl ester. In Study 2, gly­cat­ed LDL was pre­pared by gly­cat­ing LDL from 6 ­healthy indi­vid­u­als in vitro. The ­uptake of ­native LDL by mac­ro­phag­es was com­pared with that of gly­cat­ed LDL using the same meth­od as in Study 1.
­Results. In Study 1, the ­uptake of DM-LDL (­median:1,405; range: 985-2269 dpm) was sig­nif­i­cant­ly high­er than that of con­trol LDL (­median: 1,095; range: 990-1104 dpm, p<0.05). In Study 2, the ­uptake of gly­cat­ed LDL (­median: 1,556; range: 889-2837 dpm) was sig­nif­i­cant­ly high­er than that of ­native LDL (­median: 1,176; range: 789-2098 dpm, p<0.05).
Con­clu­sions. The ­results indi­cate that the great­er ­uptake of DM-LDL than that of con­trol LDL may be attrib­ut­ed to gly­ca­tion of LDL ­caused by hyper­gly­ce­mia in dia­bet­ic ­patients. Gly­ca­tion of LDL by hyper­gly­ce­mia may be one cause of the accel­er­at­ed ather­o­gen­e­sis in dia­bet­ic ­patients.

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