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Rivista di Angiologia

Official Journal of the International Union of Angiology, the International Union of Phlebology and the Central European Vascular Forum
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International Angiology 1999 June;18(2):149-53


lingua: Inglese

Impor­tance of gly­ca­tion in the accel­er­a­tion of low den­sity lip­o­pro­tein (LDL) ­uptake into mac­ro­phag­es in ­patients with dia­betes mel­lit­us

Maki­ta T., Tana­ka A., Naka­no T. *, Nakaj­i­ma K. *, Numa­no F.

From the Third Depart­ment of Inter­nal Med­i­cine, Tokyo Med­i­cal and Den­tal Uni­ver­sity, Tokyo, Japan
* Japan Immu­nor­e­search Lab­o­ra­tories, Tak­a­sa­ki City, Japan


Back­ground. Two stud­ies were con­duct­ed to deter­mine the impor­tance of gly­ca­tion in the accel­er­a­tion of low den­sity lip­o­pro­tein (LDL) ­uptake by mac­ro­phag­es in ­patients with dia­betes mel­lit­us.
Meth­ods. ­Healthy indi­vid­u­als (n=6) and non-insu­lin­de­pen­dent dia­bet­ic ­patients (n=6) were stud­ied. LDL was sep­ar­at­ed by sequen­tial ultra­cen­trif­u­ga­tion. Mac­ro­phag­es were col­lect­ed from the abdom­i­nal cav­ity of ICR-mice and incu­bat­ed in a medi­um con­tain­ing [14C] ­oleate. In Study 1, LDL from dia­bet­ic ­patients (DM-LDL) and con­trol LDL were then incu­bat­ed with this medi­um. The ­uptake of DM-LDL by mac­ro­phag­es was com­pared with that of con­trol LDL by meas­ur­ing the [14c] con­tent of the syn­the­sized cho­les­te­ryl ester. In Study 2, gly­cat­ed LDL was pre­pared by gly­cat­ing LDL from 6 ­healthy indi­vid­u­als in vitro. The ­uptake of ­native LDL by mac­ro­phag­es was com­pared with that of gly­cat­ed LDL using the same meth­od as in Study 1.
­Results. In Study 1, the ­uptake of DM-LDL (­median:1,405; range: 985-2269 dpm) was sig­nif­i­cant­ly high­er than that of con­trol LDL (­median: 1,095; range: 990-1104 dpm, p<0.05). In Study 2, the ­uptake of gly­cat­ed LDL (­median: 1,556; range: 889-2837 dpm) was sig­nif­i­cant­ly high­er than that of ­native LDL (­median: 1,176; range: 789-2098 dpm, p<0.05).
Con­clu­sions. The ­results indi­cate that the great­er ­uptake of DM-LDL than that of con­trol LDL may be attrib­ut­ed to gly­ca­tion of LDL ­caused by hyper­gly­ce­mia in dia­bet­ic ­patients. Gly­ca­tion of LDL by hyper­gly­ce­mia may be one cause of the accel­er­at­ed ather­o­gen­e­sis in dia­bet­ic ­patients.

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