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Rivista di Angiologia

Official Journal of the International Union of Angiology, the International Union of Phlebology and the Central European Vascular Forum
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International Angiology 1998 March;17(1):34-7


lingua: Inglese

Factor G path­way reac­tive activ­ity (GPRA) after sur­gery for abdom­i­nal aor­tic aneu­rysm

Sugita T. *, Watarida S., Katsuyama K., Nakajima Y., Yamamoto R., Mori A.

From the Second Department of Surgery, Shiga University of Medical Science, Otsu, Japan and * Department of Cardiovascular Surgery, Tenri Hospital, Tenri, Nara, Japan


Background. Following major sur­gery, detec­tion of endo­tox­ae­mia using the Toxicolor (Lim­u­lus) test has been report­ed. In addi­tion to endo­tox­ins, this test ­detects a reac­tant from human tis­sue, fac­tor G path­way reac­tive activ­ity (GPRA). We meas­ured endo­tox­in and GPRA in 10 ­patients dur­ing and after elec­tive sur­gery for abdom­i­nal aor­tic aneu­rysm. Additionally, we meas­ured phos­phok­i­nase (CPK) and GPRA lev­els in the mus­cle of 10 ­patients dur­ing abdom­i­nal aor­tic aneu­rysm sur­gery and 6 dur­ing open lap­a­rot­o­my for other caus­es.
Methods. Samples were taken from the arterial lines prior to sur­gery, ­before cross-clamp­ing of the aorta, imme­di­ate­ly after and 1, 3 6, and 18 hours after declamp­ing. Muscle spec­i­mens were taken from the rec­tus abdom­i­nus and homog­e­nized in many lev­els. Endotoxin con­cen­tra­tions were meas­ured with the Endotoxin-Specific test. GPRA con­cen­tra­tions were deter­mined by sub­tract­ing the val­ues from the Endotoxin-Specific test from those given by the Toxicolor test (Lim­u­lus assay with ach­rom­o­gen­ic sub­strate). CPK was also meas­ured.
Results. Endotoxin did not sig­nif­i­cant­ly ­increase dur­ing or after sur­gery, but GPRA was ele­vat­ed. GPRA in mus­cle cor­re­lat­ed sig­nif­i­cant­ly with CPK in mus­cle.
Conclusions. We con­clude that GPRA was ele­vat­ed after sur­gery, while endo­tox­in did not ­increase sig­nif­i­cant­ly. The GPRA prob­ably orig­i­nat­ed from human mus­cle tis­sue.

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