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GAZZETTA MEDICA ITALIANA ARCHIVIO PER LE SCIENZE MEDICHE

Rivista di Medicina Interna e Farmacologia


Indexed/Abstracted in: BIOSIS Previews, EMBASE, Scopus, Emerging Sources Citation Index

 

ORIGINAL ARTICLE  


Gazzetta Medica Italiana Archivio per le Scienze Mediche 2017 Gennaio-Febbraio;176(1-2):50-6

DOI: 10.23736/S0393-3660.16.03284-8

Copyright © 2015 EDIZIONI MINERVA MEDICA

lingua: Inglese

A comparison of different methods to isolating primordial germ cells

Mohsen NAEEMIPOUR

Department of Biochemistry and Nutrition, Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran


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BACKGROUND: Role of PGCs for producing transgenic chickens has significantly increased recently as a result of their exclusives capabilities. Primordial germ cells (PGCs) isolation in high numbers is the first step toward transgenic chicken production.
METHODS: In the present study we used the main methods to purify chicken PGCs including the isolating of PGCs with ammonium chloride-potassium (ACK) buffer, Ficoll and percoll density gradient centrifugation, and using the Magnetic cell sorting (MACS). We compared the methods based on the number of PGCs obtained.
RESULTS: Our results show that Ficoll density gradient centrifugation method is the preferential method.
CONCLUSIONS: There is a demand for a more suitable method for more efficient PGCs isolation.


KEY WORDS: Embryonic germ cells - Ficoll - Cell separation

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Publication History

Issue published online: November 21, 2016
Manuscript accepted: December 11, 2015
Manuscript received: November 3, 2015

Per citare questo articolo

Naeemipour M. A comparison of different methods to isolating primordial germ cells. Gazz Med Ital - Arch Sci Med 2017;176:50-6. DOI: 10.23736/S0393-3660.16.03284-8

Corresponding author e-mail

m.naeemipour@gmail.com