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GAZZETTA MEDICA ITALIANA ARCHIVIO PER LE SCIENZE MEDICHE
Rivista di Medicina Interna e Farmacologia
Indexed/Abstracted in: BIOSIS Previews, EMBASE, Scopus, Emerging Sources Citation Index
Gazzetta Medica Italiana Archivio per le Scienze Mediche 2017 January-February;176(1-2):50-6
Copyright © 2015 EDIZIONI MINERVA MEDICA
A comparison of different methods to isolating primordial germ cells
Mohsen NAEEMIPOUR ✉
Department of Biochemistry and Nutrition, Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
BACKGROUND: Role of PGCs for producing transgenic chickens has significantly increased recently as a result of their exclusives capabilities. Primordial germ cells (PGCs) isolation in high numbers is the first step toward transgenic chicken production.
METHODS: In the present study we used the main methods to purify chicken PGCs including the isolating of PGCs with ammonium chloride-potassium (ACK) buffer, Ficoll and percoll density gradient centrifugation, and using the Magnetic cell sorting (MACS). We compared the methods based on the number of PGCs obtained.
RESULTS: Our results show that Ficoll density gradient centrifugation method is the preferential method.
CONCLUSIONS: There is a demand for a more suitable method for more efficient PGCs isolation.
KEY WORDS: Embryonic germ cells - Ficoll - Cell separation