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GIORNALE ITALIANO DI DERMATOLOGIA E VENEREOLOGIA

Rivista di Dermatologia e Malattie Sessualmente Trasmesse


Official Journal of the Italian Society of Dermatology and Sexually Transmitted Diseases
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Giornale Italiano di Dermatologia e Venereologia 2017 May 16

DOI: 10.23736/S0392-0488.17.05537-7

Copyright © 2017 EDIZIONI MINERVA MEDICA

lingua: Inglese

Paraoxonases and psoriasis: negative imbalance of anti-oxidant endogenous mechanisms

Maria SCHIATTARELLA 1, Giuseppina CAIAZZO 1, Roberta DI CAPRIO 1, Serena LEMBO 2, Annunziata RAIMONDO 1, Fabio AYALA 1, Nicola BALATO 1, Giuseppe MONFRECOLA 1, Giuliana FORTUNATO 3, Anna BALATO 4

1 Department of Clinical Medicine and Surgery, University of Naples, Federico II, Naples, Italy; 2 Department of Medicine, Surgery and Dentistry “Scuola Medica Salernitana” University of Salerno, Salerno, Italy; 3 Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Naples, Italy; 4 Department of Advanced Biomedical Sciences, University of Naples Federico II, Naples, Italy


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BACKGROUNG: Numerous reports have shown that psoriasis patients are more exposed to lipoprotein peroxidation and to a decrease in the activity of paraoxonase (PON)1, an anti­oxidant and anti­inflammatory enzyme. Thus, it has been suggested that malfunction of the anti­oxidant system and an increased production of reactive oxygen species drive immune inflammatory events, that result in progressive skin cell damage in patients with psoriasis. The PON protein family, including PON1, PON2 and PON3, is one of the most important endogenous defense mechanisms against oxidative stress. In the present study, we investigated PON gene expression in psoriasis and in cutaneous oxidative stress.
METHODS: The study population included 10 patients affected by moderate­to­severe plaque psoriasis and 15 healthy donors who have undergone to plastic surgery, were used as control. Skin punch biopsies of lesional and non lesional psoriatic skin were performed for analysis of PON2 and PON3 gene expression. In addition, oxidation assays in ex vivo full­thickness healthy skin organ cultures were performed.
RESULTS: No significant differences were observed between PON2 and PON3 gene expression in psoriatic lesional and non lesional skin compared with healthy controls. H2O2 treatment induced a significant decrease of PON2 and PON3 expression in ex vivo full­thickness healthy skin organ cultures; conversely the pre­treatment of samples with the anti­oxidant reagent N­acetyl­L­cysteine (NAC) induced a significant increase. Interestingly, no significant alterations were reported for PON2 and PON3 expression in ex vivo full­thickness healthy skin organ cultures stimulated with IL­17.
CONCLUSIONS: Taken together our findings have revealed that a strong pro­oxidative activity is not effectively countered by anti­oxidant endogenous mechanisms both in psoriatic skin and in ex vivo experimental model.


KEY WORDS: PON2­ - PON3 -­ Psoriasis­ - Oxidative stress

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Schiattarella M, Caiazzo G, Di Caprio R, Lembo S, Raimondo A, Ayala F, et al. Paraoxonases and psoriasis: negative imbalance of anti-oxidant endogenous mechanisms. G Ital Dermatol Venereol 2017 May 16. DOI: 10.23736/S0392-0488.17.05537-7 

Corresponding author e-mail

annabalato@yahoo.it