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Rivista di Chirurgia
Indexed/Abstracted in: EMBASE, Scopus, Emerging Sources Citation Index
Chirurgia 2014 June;27(3):143-9
Use of Shigyaku-san to improve liver function and regeneration after hepatectomy in rats
Wu H., Iwata H., Kimura M., Sekino T., Yamada T., Takemura H.
Department of General and Cardiothoracic Surgery, Graduate School of Medicine, Gifu University, Gifu, Japan
AIM: Shigyaku-san is known to alleviate the effects of experimental liver damage. The aim of this study was to clarify the effects of Shigyaku-san on liver function and regeneration after hepatectomy in rats.
METHODS: Rats were divided into three groups (control, Shigyaku-san, no-hepatectomy). Blood biochemistry was analyzed at 2, 6, 24, and 72 h postoperatively. The remnant liver, liver-body weight ratio and immunohistochemistry stains were evaluated before hepatectomy and at 24 and 72 h postoperatively. Serum IL-6 and TNF-α were measured before hepatectomy and 2 and 6 h postoperatively.
RESULTS: Serum ALT and T-Chol were significantly lower in the Shigyaku-san group than in the control group at 24 and 72 h postoperatively, and serum LDH was significantly lower 6 h postoperatively. Regenerated liver weight was significantly increased in the Shigyaku-san group compared with the control group at 72 h postoperatively. The proliferating cell nuclear antigen (PCNA) labeling index (LI) and liver-body weight ratio were significantly higher in the Shigyaku-san group than in the control group 24 and 72 h postoperatively. Ki67 was significantly higher in the Shigyaku-san group than in the control group 72 h postoperatively. Compared with the control group, serum IL-6 was significantly increased 2 h postoperatively, but significantly decreased 6 h postoperatively in the Shigyaku-san group.
CONCLUSION: These data suggest that Shigyaku-san protects against liver damage and promotes liver regeneration after partial hepatectomy in rats.