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The Journal of Cardiovascular Surgery 2002 December;43(6):891-4

Copyright © 2009 EDIZIONI MINERVA MEDICA

lingua: Inglese

In vivo evaluation of lumbrokinase, a fibrinolytic enzyme extracted from Lumbricus rubellus, in a prosthetic vascular graft

Hwang C. M. 1, Kim D. I. 2, Kim J. E. 2, Huh S. H. 2, Min B. G. 1, Park J. H. 2, Han J. S. 2, Lee B. B. 2, Kim Y. I. 2, Ryu E. S. 1, Kim J. W. 1

1 Department of Biomedical Engineering, College of Engineering, Seoul National University, Seoul, Korea 2 Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea


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Back­ground. Lum­bro­kinase (LK) is a fibrin­o­lytic ­enzyme pur­i­fied ­from the ­earth­worm Lum­bricus ­rubellus. To inves­ti­gate the fibrin­o­lytic and anti­throm­botic ­effects of lum­brok­i­nase, a ­series of ­animal experi­ments ­were per­formed.
­Methods. The ­Dacron ­graft (3 mm in diam­eter, 3 cm in ­length) ­were ­treated ­with LK via two dif­ferent ­methods, ­simple dip­ping and cova­lent ­bonding ­methods. Cova­lent ­bonding was per­formed by UV reac­tion to poly­acrylic ­acid. The ­grafts ­were inter­posed ­into the infe­rior ­vena ­cava of the rab­bits and har­vested for 5 ­hours, 1, 2 and 4 ­weeks ­after the implan­ta­tion.
­Results. The LK non-­treated ­graft (n=4) ­were ­totally ­occluded ­with ­thrombus 5 ­hours ­after the implan­ta­tion. ­Both ­types of LK ­treated ­graft (n=8) ­were ­patent 1 ­week ­after the implan­ta­tion. The ­grafts ­treated ­with the ­simple dip­ping ­method (n=4) ­were ­occluded ­with ­thrombus 2 ­weeks ­after the implan­ta­tion. The ­grafts ­treated ­with cova­lent ­bonding (n=4) ­were ­patent 4 ­weeks ­after the implan­ta­tion. Ultra­struc­tural anal­ysis of the ­luminal sur­face of the ­patent ­grafts by scan­ning elec­tron micros­copy ­revealed the ­thin ­plasma pro­tein ­layer to be ­about 5 ­μ in thick­ness ­with ­platelet adhe­sions.
Con­clu­sions. Lum­brok­i­nase has poten­tial anti­throm­botic ­effects in a ­small diam­eter vas­cular pros­thesis. The cova­lent ­bonding ­method ­proved to be ­more effec­tive ­than the ­simple dip­ping ­method.

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