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THE JOURNAL OF CARDIOVASCULAR SURGERY
Rivista di Chirurgia Cardiaca, Vascolare e Toracica
Indexed/Abstracted in: BIOSIS Previews, Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
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ORIGINAL ARTICLES VASCULAR SECTION
The Journal of Cardiovascular Surgery 2002 December;43(6):891-4
In vivo evaluation of lumbrokinase, a fibrinolytic enzyme extracted from Lumbricus rubellus, in a prosthetic vascular graft
Hwang C.-M. 1, Kim D.-I. 2, Kim J.-E. 2, Huh S.-H. 2, Min B.-G. 1, Park J.-H. 2, Han J.-S. 2, Lee B.-B. 2, Kim Y.-I. 2, Ryu E.-S. 1, Kim J.-W. 1
1 Department of Biomedical Engineering, College of Engineering, Seoul National University, Seoul, Korea
2 Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
Background. Lumbrokinase (LK) is a fibrinolytic enzyme purified from the earthworm Lumbricus rubellus. To investigate the fibrinolytic and antithrombotic effects of lumbrokinase, a series of animal experiments were performed.
Methods. The Dacron graft (3 mm in diameter, 3 cm in length) were treated with LK via two different methods, simple dipping and covalent bonding methods. Covalent bonding was performed by UV reaction to polyacrylic acid. The grafts were interposed into the inferior vena cava of the rabbits and harvested for 5 hours, 1, 2 and 4 weeks after the implantation.
Results. The LK non-treated graft (n=4) were totally occluded with thrombus 5 hours after the implantation. Both types of LK treated graft (n=8) were patent 1 week after the implantation. The grafts treated with the simple dipping method (n=4) were occluded with thrombus 2 weeks after the implantation. The grafts treated with covalent bonding (n=4) were patent 4 weeks after the implantation. Ultrastructural analysis of the luminal surface of the patent grafts by scanning electron microscopy revealed the thin plasma protein layer to be about 5 μ in thickness with platelet adhesions.
Conclusions. Lumbrokinase has potential antithrombotic effects in a small diameter vascular prosthesis. The covalent bonding method proved to be more effective than the simple dipping method.