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Rivista di Chirurgia Cardiaca, Vascolare e Toracica

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The Journal of Cardiovascular Surgery 2001 December;42(6):753-7

lingua: Inglese

Smooth muscle cell proliferation at the vascular anastomotic stricture in rat aortotomy model

Ogata T., Kurabayashi M. *, Hoshino Y. *, Sato Y., Kano M., Ohki Sa., Ishikawa S., Morishita Y., Nagai R. **

From the Second Department of Surgery
*Second Department of Internal Medicine Gunma University School of Medicine, Gunma, Japan
**Department of Cardiovascular Internal Medicine Tokyo University School of Medicine, Tokyo, Japan


Background. We recent­ly sug­gest­ed ­that a rat aor­tot­o­my mod­el ­could be sub­sti­tut­ed for a vas­cu­lar anas­to­mot­ic stric­ture ­around a ­suture ­line. The aim of ­this ­study was to ver­i­fy ­such a rat aor­tot­o­my mod­el ­using pro­life­rat­ing ­cell nucle­ar anti­gen (­PCNA) immu­no­his­to­chem­is­try, ­which is a sen­si­tive meth­od for detec­tion of pro­life­rat­ing ­cells in vas­cu­lar tis­sue ­after inju­ry.
Methods. Longitudinal aor­tot­o­my was per­formed in the abdom­i­nal aor­ta of the rat sub­jects. The ­rats ­were sac­ri­ficed at 1, 2, 4 and 8 ­weeks fol­low­ing aor­tot­o­my (n=20 in ­each ­group). The con­trol ­rats ­were sac­ri­ficed with­out aor­tot­o­my (n=20). The per­cent­age of the ­lumen occlud­ed by inti­mal thick­en­ing (I/M ­ratio) was cal­cu­lat­ed. All tis­sues ­were ­stained ­with anti­bod­ies ­against pro­life­rat­ing ­cell nucle­ar anti­gen (­PCNA).
Results. Values of I/M ­ratio ­were 8.5±4.4%, 15.6±9.5%, 11.8±5.5%, 9.6±6.2% at 1, 2, 4, 8 ­weeks fol­low­ing aor­tot­o­my in the ­injured ­groups and 0.4±0.1% in the con­trols, respec­tive­ly. Those val­ues in the ­injured ­group ­increased sig­nif­i­cant­ly as com­pared to the con­trols. There ­were ­also sig­nif­i­cant dif­fer­enc­es ­between one ­week and two ­weeks fol­low­ing aor­tot­o­my. The ­PCNA label­ing ­index at one ­week fol­low­ing aor­tot­o­my (21.4±2.7%) was sig­nif­i­cant­ly high­er ­than at two ­weeks fol­low­ing aor­tot­o­my (2.8±1.9%). SMCs in the inti­ma at four and eight ­weeks fol­low­ing aor­tot­o­my ­were com­plete­ly neg­a­tive for ­PCNA.
Conclusions. The experi­men­tal rat aor­tot­o­my mod­el was deter­mined to be use­ful in the inves­ti­ga­tion of inti­mal thick­en­ing ­around the ­suture ­line.

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