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THE JOURNAL OF CARDIOVASCULAR SURGERY

Rivista di Chirurgia Cardiaca, Vascolare e Toracica


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The Journal of Cardiovascular Surgery 2000 August;41(4):529-32

Copyright © 2009 EDIZIONI MINERVA MEDICA

lingua: Inglese

Neurogenic vasoreactivity of human internal thoracic artery is unaffected by endothelial cell integrity

Canver C. C., Cooler S. D., Saban R.

From the Division of Cardiothoracic Surgery Albany Medical College, Albany, New York *Smooth Muscle Laboratory University of Wisconsin School of Veterinary Medicine Madison, Wisconsin, USA


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Back­ground. The ­role of ­internal tho­racic ­artery (ITA) ner­vous ­supply has not ­been pre­vi­ously con­sid­ered as a poten­tial ­factor influ­encing excel­lent ­long-­term ­patency of an ITA ­graft. To ­define the inter­ac­tion ­between the pri­mary ­afferent neu­rons and endo­the­lial ­cells of ITA, we inves­ti­gated the ­effects of ­acute cap­sa­icin admin­is­tra­tion in ­vitro on the iso­metric ten­sion of ­human ­ITAs.
­Methods. Ves­sels ­were ­obtained ­from ­patients under­going cor­o­nary ­bypass or ­from ­multi-­organ trans­plant ­donors. ­Thirty-­three ITA seg­ments (5 mm ­wide) ­were sus­pended as ­rings ­between two stain­less-­steel stir­rups in ­water-jack­eted (37°C) ­tissue ­baths. The ­tissue ­baths con­tained 10 ml phys­io­log­ical ­salt solu­tion (PSS) of the fol­lowing com­po­si­tion (mM/L): NaC1 119, KC1 4.7, NaH2PO4 1.0, ­MgCI2 0.5, CaC12 2.5, ­NaHCO3 25, and glu­cose 11, aer­ated con­tin­u­ously ­with 95% O2 and 5% CO2. Pep­ti­dase inhib­i­tors, phos­pho­ram­idon (1 μM) and cap­to­pril (1 μM), ­were ­added to PSS to ­decrease pep­tide deg­ra­da­tion. Mechan­ical ­responses ­were meas­ured iso­met­ri­cally and ­recorded on a poly­graph via iso­metric ­force trans­ducers. Ves­sels ­were pre­con­stricted ­with sub­max­imal con­cen­tra­tions of nor­epi­neph­rine. ­After the ten­sion had sta­bi­lized, cap­sa­icin was ­added cumu­la­tively to the ­tissue ­bath. The ­viability of ITA was ver­i­fied by its ­responses to endo­the­lial-depen­dent (ace­tyl­cho­line, 1 μM) (n=20) and endo­the­lial-inde­pen­dent (­sodium nitro­prus­side, 10 μM) (n=13) vaso­di­la­tors.
­Results. The expo­sure of cap­sa­icin (3 μM) to ­human ITA pro­duced ­varied ­effects on ITA irre­spec­tive of its endo­the­lium. Cap­sa­icin ­induced con­trac­tion of the ITA ­smooth ­muscle in 13 endo­the­lium-­intact ITA seg­ments ­while it pro­duced vaso­con­stric­tion in 9 endo­the­lium-­denuded ­ITAs (p=0.6437). In ­response to cap­sa­icin, relax­a­tion of ITA ­smooth ­muscle was ­observed in 7 ITA ­rings ­with endo­the­lium, ­while vasod­i­la­tion was ­present in 4 ITA seg­ments ­without endo­the­lium (p=0.4099).
Con­clu­sions. Cap­sa­icin-sen­si­tive neu­rons encir­cling ­human ITA pro­duce a neu­ro­genic vas­o­reac­tive ­response inde­pen­dent of ITA endo­the­lial ­cell integ­rity.

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