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Rivista di Chirurgia Cardiaca, Vascolare e Toracica

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The Journal of Cardiovascular Surgery 2000 August;41(4):529-32

lingua: Inglese

Neurogenic vasoreactivity of human internal thoracic artery is unaffected by endothelial cell integrity

Canver C. C., Cooler S. D., Saban R. *

From the Divi­sion of Car­di­oth­o­racic Sur­gery Albany Med­ical Col­lege, ­Albany, New ­York
*­Smooth ­Muscle Labor­a­tory Uni­ver­sity of Wis­consin ­School of Vet­e­ri­nary Med­i­cine Mad­ison, Wis­consin, USA


Back­ground. The ­role of ­internal tho­racic ­artery (ITA) ner­vous ­supply has not ­been pre­vi­ously con­sid­ered as a poten­tial ­factor influ­encing excel­lent ­long-­term ­patency of an ITA ­graft. To ­define the inter­ac­tion ­between the pri­mary ­afferent neu­rons and endo­the­lial ­cells of ITA, we inves­ti­gated the ­effects of ­acute cap­sa­icin admin­is­tra­tion in ­vitro on the iso­metric ten­sion of ­human ­ITAs.
­Methods. Ves­sels ­were ­obtained ­from ­patients under­going cor­o­nary ­bypass or ­from ­multi-­organ trans­plant ­donors. ­Thirty-­three ITA seg­ments (5 mm ­wide) ­were sus­pended as ­rings ­between two stain­less-­steel stir­rups in ­water-jack­eted (37°C) ­tissue ­baths. The ­tissue ­baths con­tained 10 ml phys­io­log­ical ­salt solu­tion (PSS) of the fol­lowing com­po­si­tion (mM/L): NaC1 119, KC1 4.7, NaH2PO4 1.0, ­MgCI2 0.5, CaC12 2.5, ­NaHCO3 25, and glu­cose 11, aer­ated con­tin­u­ously ­with 95% O2 and 5% CO2. Pep­ti­dase inhib­i­tors, phos­pho­ram­idon (1 μM) and cap­to­pril (1 μM), ­were ­added to PSS to ­decrease pep­tide deg­ra­da­tion. Mechan­ical ­responses ­were meas­ured iso­met­ri­cally and ­recorded on a poly­graph via iso­metric ­force trans­ducers. Ves­sels ­were pre­con­stricted ­with sub­max­imal con­cen­tra­tions of nor­epi­neph­rine. ­After the ten­sion had sta­bi­lized, cap­sa­icin was ­added cumu­la­tively to the ­tissue ­bath. The ­viability of ITA was ver­i­fied by its ­responses to endo­the­lial-depen­dent (ace­tyl­cho­line, 1 μM) (n=20) and endo­the­lial-inde­pen­dent (­sodium nitro­prus­side, 10 μM) (n=13) vaso­di­la­tors.
­Results. The expo­sure of cap­sa­icin (3 μM) to ­human ITA pro­duced ­varied ­effects on ITA irre­spec­tive of its endo­the­lium. Cap­sa­icin ­induced con­trac­tion of the ITA ­smooth ­muscle in 13 endo­the­lium-­intact ITA seg­ments ­while it pro­duced vaso­con­stric­tion in 9 endo­the­lium-­denuded ­ITAs (p=0.6437). In ­response to cap­sa­icin, relax­a­tion of ITA ­smooth ­muscle was ­observed in 7 ITA ­rings ­with endo­the­lium, ­while vasod­i­la­tion was ­present in 4 ITA seg­ments ­without endo­the­lium (p=0.4099).
Con­clu­sions. Cap­sa­icin-sen­si­tive neu­rons encir­cling ­human ITA pro­duce a neu­ro­genic vas­o­reac­tive ­response inde­pen­dent of ITA endo­the­lial ­cell integ­rity.

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