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ULTIMO FASCICOLOTHE JOURNAL OF CARDIOVASCULAR SURGERY

Rivista di Chirurgia Cardiaca, Vascolare e Toracica

Indexed/Abstracted in: BIOSIS Previews, Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 1,632

Periodicità: Bimestrale

ISSN 0021-9509

Online ISSN 1827-191X

 

The Journal of Cardiovascular Surgery 2000 Aprile;41(2):171-4

CARDIAC PAPERS 

 ORIGINAL ARTICLES

Association of osteopontin with calcification in human mitral valves

Canver C. C., Gregory R. D., Cooler S. D., Voytovich M. C. *

From the Divi­sion of Car­di­oth­o­racic Sur­gery Albany Med­ical Col­lege, ­Albany, New ­York
*Depart­ment of Path­o­log­y U­ni­ver­sity of Wis­consin ­School of Med­i­cine Mad­ison, Wis­consin, USA

Back­ground. ­Little is ­known ­about the ­long-­standing inflam­ma­tion ­leading to cal­cifi­ca­tion ­within ­heart ­valves. Oste­o­pontin, a phos­phor­y­lated gly­co­pro­tein, is ­present ­within ath­e­rom­a­tous cal­cific ­plagues in ­response to vas­cular endo­the­lial ­injury. The pur­pose of the ­study was to inves­ti­gate ­whether oste­o­pontin ­exists in cal­cific ­mitral ­valve leaf­lets in ­human ­beings, and to deter­mine a ­link ­between ­chronic inflam­ma­tion ­leading to ­mitral sten­osis and the oste­o­pontin ­status of ­mitral val­vular ­tissue.
Mate­rials and ­methods. We ­reviewed the ­case his­to­ries of 17 ­patients who under­went ­mitral ­valve replace­ment ­therapy for ­mitral sten­osis ­between 1995 and 1997 (8 men and 9 ­women, ­mean age 61 ­years). ­Hybrid ­mouse mono­clonal immu­no­glob­ulin G1 anti­bodies ­were ­used for immu­no­his­to­chem­ical detec­tion of oste­o­pontin in the ace­tone-­fixed spec­imen. The con­trol ­group con­sisted of ­normal ­mitral ­valve ­tissue ­from car­di­om­yo­pathy ­patients who under­went car­diac trans­plan­ta­tion.
­Results. A ­weak oste­o­pontin immu­no­reac­tivity was ­present in appar­ently ­normal ­mitral ­valve ­tissue ­obtained ­from car­di­om­yo­pathy ­patients. All ­mitral sten­osis ­patients had immu­no­reac­tivity (17/17) for oste­o­pontin ­within cal­cific ­deposits of ­mitral ­valve ­tissue. The inten­sity of oste­o­pontin ­activity had a ­strong asso­ci­a­tion ­with ­increasing mac­ro­phage and cal­cium aggre­ga­tions in the ­mitral val­vular ­tissue. We ­found no cor­re­la­tion ­between oste­o­pontin ­status and clin­ical fea­tures on the prog­nosis of cal­cific ­mitral sten­osis.
Con­clu­sions. We con­clude ­that oste­o­pontin coex­ists ­with ­intimal mac­ro­phages in cal­cific ­human ­mitral ­valve ­tissue. Dem­on­stra­tion of ­such asso­ci­a­tion ­between the pres­ence of oste­o­pontin and cal­cifi­ca­tion in ­human ­mitral ­valves is con­sis­tent ­with the hypoth­esis ­that cal­cifi­ca­tion in ­this ­tissue is, at ­least in ­part, an ­actively medi­ated phe­nom­enon.

lingua: Inglese


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