Home > Riviste > The Journal of Cardiovascular Surgery > Fascicoli precedenti > The Journal of Cardiovascular Surgery 1998 June;39(3) > The Journal of Cardiovascular Surgery 1998 June;39(3):321-9

ULTIMO FASCICOLO
 

ARTICLE TOOLS

Estratti

THE JOURNAL OF CARDIOVASCULAR SURGERY

Rivista di Chirurgia Cardiaca, Vascolare e Toracica


Indexed/Abstracted in: BIOSIS Previews, Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 2,179


eTOC

 

ORIGINAL ARTICLES  CARDIAC PAPERS


The Journal of Cardiovascular Surgery 1998 June;39(3):321-9

Copyright © 2000 EDIZIONI MINERVA MEDICA

lingua: Inglese

Cardioprotective effect of L-arginine in myocardial ischemia and reperfusion in an isolated working rat heart model

Izhar U., Schwalb H., Borman J. B., Merin C.

From the Cardiothoracic Surgery Department and Joseph Lunenfeld Cardiac Surgery Research Center Hadassah University Hospital, Jerusalem, Israel


PDF  


Background. Studies in myo­car­dial ische­mia ­and reper­fu­sion ­have dem­on­strat­ed dam­age to endo­the­li­um, ­impaired pro­duc­tion ­and ­release of vasoac­tive sub­stanc­es ­such as ­nitric ­oxide, ­and ­marked alter­a­tion in endo­the­li­um-depen­dent relax­atin of ­the cor­o­nary vas­cu­la­ture. This ­study ­was ­designed to exam­ine ­the car­diop­ro­tec­tive ­effect of ex­og­e­nous admin­is­tra­tion of L-argi­nine, a pre­cur­sor of ­nitric ­oxide, dur­ing ische­mia ­and reper­fu­sion, par­tic­u­lar­ly ­using oxy­gen­at­ed crys­tal­loid car­di­o­ple­gia.
Methods. Seventy ener­gy-deplet­ed iso­lat­ed work­ing ­rat ­hearts ­were arrest­ed by car­di­o­ple­gia ­and sub­ject­ed to 60 ­min norm­other­mic glo­bal ische­mia fol­lowed by 10 ­min non­work­ing ­and 30 ­min work­ing reper­fu­sion (Gr 1). L-argi­nine (3mM or 10mM) ­was add­ed to ­the car­di­o­pleg­ic solu­tion (Gr 2,3 respec­tive­ly), reper­fu­sion (Gr 6,7 respec­tive­ly), through­out ­the experi­ment (Gr 4,5 respec­tive­ly), ­and ­with Nw-­nitro-L-argi­nine meth­yl ­ester (L-­NAME), a com­pet­i­tive inhib­i­tor of ­nitric ­oxide syn­thase (Gr 8).
Results. At 30 ­min of work­ing ­heart reper­fu­sion, com­pared to con­trol, ­all argi­nine con­tain­ing ­groups (Gr 2-7) exhib­it­ed a sig­nif­i­cant ­improved recov­ery of car­diac out­put (64.7±21.2, 98.1±21.1, 90.9±11.7, 88.9±16.2, 83.1±7.4, ­and 90.8±10.6, ­mean ± SD% Gr 2 to 7 respec­tive­ly, vs Gr 1 36.3±20%, p<0.01). Significant recov­ery improve­ment ­was ­observed ­also in oth­er hemo­dy­nam­ic param­e­ters (cor­o­nary ­flow, aor­tic ­peak pres­sure), as ­well as bio­chem­i­cal recov­ery ­assessed by O2 con­sump­tion ­ratio, ­release of lac­tic dehy­drog­e­nase at reper­fu­sion ­and regen­er­a­tion of ­ATP. The L-­NAME ­group ­had a sig­nif­i­cant poor­er hemo­dy­nam­ic ­and bio­chem­i­cal recov­ery. L-argi­nine ­had no ­effect on ­the pre­is­chem­ic hemo­dy­nam­ic param­e­ters.
Conclusions. These ­results sup­port ­the cumu­la­tive ­data con­sid­er­ing L-argi­nine as a car­di­op­ro­tec­tive ­agent in post­is­chem­ic reper­fu­sion inju­ry.

inizio pagina

Publication History

Per citare questo articolo

Corresponding author e-mail