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FASCICOLI E ARTICOLI   I PIÙ LETTI   eTOC

ULTIMO FASCICOLOTHE JOURNAL OF CARDIOVASCULAR SURGERY

Rivista di Chirurgia Cardiaca, Vascolare e Toracica

Indexed/Abstracted in: BIOSIS Previews, Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 1,632

Periodicità: Bimestrale

ISSN 0021-9509

Online ISSN 1827-191X

 

The Journal of Cardiovascular Surgery 1998 Giugno;39(3):321-9

CARDIAC PAPERS 

 ORIGINAL ARTICLES

Cardioprotective ­effect of L-argi­nine in myo­car­dial ische­mia ­and reper­fu­sion in an iso­lat­ed work­ing ­rat ­heart mod­el

Izhar U., Schwalb H., Borman J. B., Merin C.

From ­the Cardiothoracic Surgery Department and Joseph Lunenfeld Cardiac Surgery Research Center Hadassah University Hospital, Jerusalem, Israel

Background. Studies in myo­car­dial ische­mia ­and reper­fu­sion ­have dem­on­strat­ed dam­age to endo­the­li­um, ­impaired pro­duc­tion ­and ­release of vasoac­tive sub­stanc­es ­such as ­nitric ­oxide, ­and ­marked alter­a­tion in endo­the­li­um-depen­dent relax­atin of ­the cor­o­nary vas­cu­la­ture. This ­study ­was ­designed to exam­ine ­the car­diop­ro­tec­tive ­effect of ex­og­e­nous admin­is­tra­tion of L-argi­nine, a pre­cur­sor of ­nitric ­oxide, dur­ing ische­mia ­and reper­fu­sion, par­tic­u­lar­ly ­using oxy­gen­at­ed crys­tal­loid car­di­o­ple­gia.
Methods. Seventy ener­gy-deplet­ed iso­lat­ed work­ing ­rat ­hearts ­were arrest­ed by car­di­o­ple­gia ­and sub­ject­ed to 60 ­min norm­other­mic glo­bal ische­mia fol­lowed by 10 ­min non­work­ing ­and 30 ­min work­ing reper­fu­sion (Gr 1). L-argi­nine (3mM or 10mM) ­was add­ed to ­the car­di­o­pleg­ic solu­tion (Gr 2,3 respec­tive­ly), reper­fu­sion (Gr 6,7 respec­tive­ly), through­out ­the experi­ment (Gr 4,5 respec­tive­ly), ­and ­with Nw-­nitro-L-argi­nine meth­yl ­ester (L-­NAME), a com­pet­i­tive inhib­i­tor of ­nitric ­oxide syn­thase (Gr 8).
Results. At 30 ­min of work­ing ­heart reper­fu­sion, com­pared to con­trol, ­all argi­nine con­tain­ing ­groups (Gr 2-7) exhib­it­ed a sig­nif­i­cant ­improved recov­ery of car­diac out­put (64.7±21.2, 98.1±21.1, 90.9±11.7, 88.9±16.2, 83.1±7.4, ­and 90.8±10.6, ­mean ± SD% Gr 2 to 7 respec­tive­ly, vs Gr 1 36.3±20%, p<0.01). Significant recov­ery improve­ment ­was ­observed ­also in oth­er hemo­dy­nam­ic param­e­ters (cor­o­nary ­flow, aor­tic ­peak pres­sure), as ­well as bio­chem­i­cal recov­ery ­assessed by O2 con­sump­tion ­ratio, ­release of lac­tic dehy­drog­e­nase at reper­fu­sion ­and regen­er­a­tion of ­ATP. The L-­NAME ­group ­had a sig­nif­i­cant poor­er hemo­dy­nam­ic ­and bio­chem­i­cal recov­ery. L-argi­nine ­had no ­effect on ­the pre­is­chem­ic hemo­dy­nam­ic param­e­ters.
Conclusions. These ­results sup­port ­the cumu­la­tive ­data con­sid­er­ing L-argi­nine as a car­di­op­ro­tec­tive ­agent in post­is­chem­ic reper­fu­sion inju­ry.

lingua: Inglese


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