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ULTIMO FASCICOLOTHE JOURNAL OF CARDIOVASCULAR SURGERY

Rivista di Chirurgia Cardiaca, Vascolare e Toracica


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The Journal of Cardiovascular Surgery 1998 Aprile;39(2):193-9

CARDIAC PAPERS 

 ORIGINAL ARTICLES

Papaverine solu­tions ­cause ­loss of viab­i­liy­ of endo­the­lial ­cells

Rubens F. D., Labow R. S.*, Meek E., Bedard E., Gill I. S., Dudani A. K.*, Ganz P. R.*

From ­the Department of Surgery Heart Institute ­and *Department of Biochemistry Faculty of Medicine, University of Ottawa Ottawa, Ontario, Canada

Objective. The opti­mal com­po­si­tion of ­the solu­tion ­used ­for irri­ga­tion of saph­e­nous ­veins ­used ­for car­diac sur­gery ­may influ­ence ulti­mate ­graft paten­cy ­due to poten­tial inju­ri­ous ­effects on ­the ­vein endo­the­li­um of ­some of ­the solu­tion con­stit­u­ents.
Experimental ­design. The viabil­ity of cul­tured saph­e­nous ­vein endo­the­lial ­cells ­was ­assessed ­after incu­ba­tion of saph­e­nous ­vein endo­the­lial ­cells ­with solu­tions con­tain­ing ­saline, ­saline ­with papav­e­rine (0.15 M NaCl, 32.5 mg/mL papav­e­rine), cul­ture medi­um ­and buf­fered ­saline solu­tion (Plasma-Lyte-A).
Results. Cell viabil­ity ­was sig­nif­i­cant­ly ­decreased ­after ­one ­hour incu­ba­tion ­with solu­tions con­tan­ing ­saline ­with papav­e­rine (24.4±9.4%) as com­pared to cul­ture medi­um ­and buf­fered ­saline solu­tions (medi­um 100%, Plasma-Lyte-A 86.8±6.9%). Loss of viabil­ity ­was direct­ly relat­ed to ­the ­length of expo­sure of ­the cul­tured ­cells to papav­e­rine. Morphologic chang­es of ­cells incu­bat­ed ­with ­saline: papav­e­rine ­were ­also ­seen includ­ing ­cell retrac­tion ­and nucle­ar pyk­no­sis. The ­cells ­exposed to medi­um recov­ered 100% viabil­ity where­as by 4 ­hours ­only 22% of ­the ­saline: papav­e­rine ­cells ­were ­viable, ­and by 3 ­days ­this viabil­ity ­had fal­len to 7.7%.
Conclusions. Loss of viabil­ity ­was ­shown in cul­tured saph­e­nous ­vein endo­the­lial ­cells ­exposed to ­saline solu­tions con­tain­ing papav­e­rine, where­as no dif­fer­ence ­was ­found ­between cul­ture medi­um, ­saline ­and bal­anced ­salt solu­tions. Cell ­death ­was direct­ly relat­ed to ­the ­length of expo­sure of ­the ­cells to papav­e­rine. Further, ­after ­short- ­and ­long-­term recov­ery peri­ods, ­there ­was lit­tle recov­ery of ­cell viabil­ity. Although papav­e­rine is a ­potent vaso­di­la­tor, expo­sure to ­this com­pound ­may com­pro­mise ­long-­term viabil­ity of ­graft endo­the­lial ­cells.

lingua: Inglese


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