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ITALIAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY

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Giornale Italiano di Chirurgia Vascolare 1999 June;6(2):105-18

Copyright © 2000 EDIZIONI MINERVA MEDICA

language: English, Italian

A comparative morphological and functional study of the effects of UW and Celsior solution in the hypothermic preservation of rat aorta

Nardo B., Catena F., Cavallari G., Turi P., De Vincentis F., Giordano R. °, Pasquinelli G. *, Faenza A., Cavallari A.

From the Surgical Clinic II (Head: Prof. A. Cavallari) * Department of Biochemistry (Head: Prof. L. Masotti) ** Institute of Electron Microscopy (Head: Prof. M. Derenzini) University of Bologna, Bologna, Italy


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Background and aim. Few and con­tro­ver­sial ­data are report­ed in the lit­er­a­ture regard­ing the ­effects of endo­the­li­um-medi­ated vas­o­reg­u­la­tion of solu­tions ­with dif­fer­ing potas­sium con­tents. A num­ber of ­works ­have ­shown ­that low potas­sium con­cen­tra­tions are ­more effec­tive for the ­short- and ­long-­term pres­er­va­tion of arter­ies. Other stud­ies ­have sug­gest­ed ­that the ­high K+ con­tent of UW solu­tion ­might ­alter the bal­ance ­between the dif­fer­ent vasoac­tive sub­stanc­es pro­duced by vas­cu­lar endo­the­li­um. Endothelial ­cells ­release vasoac­tive sub­stanc­es ­that exer­cise the ­main ­effects on ­local vas­o­reg­u­la­tion. These ­include the capac­ity to ­release ­smooth mus­cle ­cells and the inhi­bi­tion of plate­let adhe­sion and aggre­gra­tion. The aim of ­this ­study was to eval­u­ate chang­es in the mor­phol­o­gy and func­tion of rat aor­ta sam­ples pre­served at 4°C for var­y­ing peri­ods of ­time in two solu­tions: one ­with a ­high potas­sium con­cen­tra­tion (UW) and the oth­er ­with a low potas­sium con­tent (Celsior).
Methods. Male Wistar ­rats ­were ­used, weigh­ing ­between 220-250 ­grams, ­kept in stan­dard con­di­tions and fed on a ­free ­diet. Under gen­er­al anes­the­sia ­with ­ether, the tho­rac­ic aor­ta was ­removed, ­after lav­age in ­situ ­through the abdom­i­nal aor­ta ­with 10 cc of UW or Celsior solu­tion. Morphological anal­y­sis was per­formed ­using a scan­ning elec­tron micro­scope (SEM) and trans­mis­sion elec­tron micro­scope (TEM). Vasomotor func­tion was eval­u­at­ed by record­ing the iso­met­ric ten­sion of the aor­tic seg­ments ­exposed to adre­ner­gic and acet­yl­chol­i­ner­gic stim­u­li, respec­tive­ly con­tract­ing and dil­a­tat­ing the ves­sel. The ­results ­obtained ­were ana­lysed ­using Student’s “t” ­test for ­unpaired ­data and var­i­ance anal­y­sis fol­lowed by Bonferroni’s ­post -test.
Results. No sig­nif­i­cant mor­pho­func­tion­al dif­fer­ence was ­observed in the aor­ta seg­ments pre­served in ­either solu­tion for 48 ­hours. On the con­trary, great­er mor­pho­log­i­cal and func­tion­al alter­a­tions ­were ­found ­after 72 ­hours in aor­ta seg­ments pre­served at 4°C in UW com­pared to ­those ­kept in Celsior solu­tion.
Conclusions. For rel­a­tive­ly ­long peri­ods (72 ­hours), Celsior solu­tion, ­with a low potas­sium con­cen­tra­tion, ­should be pre­ferred to hyper­po­tas­sic UW solu­tion ­because it is ­more effec­tive in pro­tect­ing endo­the­lial func­tion and the reac­tiv­ity of the ­smooth mus­cle com­po­nent.

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