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ITALIAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY

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Giornale Italiano di Chirurgia Vascolare 1998 December;5(4):237-46

language: English, Italian

A com­par­a­tive ­study of simul­ta­ne­ous deter­mi­na­tion by cap­il­lary ­zone elec­tro­pho­re­sis and ­high per­for­mance liq­uid chrom­a­tog­ra­phy of allan­toin, ­uric ­acid, hypox­an­thine and xan­thine in ath­e­ros­cle­rot­ic ­plaque

Setacci C. *, Giubbolini M. *, Cappelli A. *, Palasciano G., Arezzini L., Landini T. *, Porcelli B., Terzuoli L.

From the * Cattedra di Chirurgia Vascolare Istituto di Biochimica e di Enzimologia Università ­degli Studi, Siena, Italy


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Background. Allantoin, ­uric ­acid (UA), hypox­an­thine (Hx) and xan­thine (X) are ­enhanced dur­ing oxi­da­tive ­stress. We ­want deter­mine ­these metab­olites in ath­e­ros­cle­rot­ic ­plaque, in ­which oxi­da­tive ­stress is ­known, to ­improve our knowl­edge on ath­ero­scler­o­sis and to ­give a fur­ther ­help in the ­plaque char­ac­ter­iza­tion. In ­this prel­i­minary ­work, we pro­pose a com­par­a­tive ­study for the simul­ta­ne­ous deter­mi­na­tion of ­these com­pounds by cap­il­lary ­zone elec­tro­pho­re­sis (CE) and ­high per­for­mance liq­uid chrom­a­tog­ra­phy (­HPLC), to eval­u­ate the meth­od ­most suit­able for ­this anal­y­sis. This ­study is ­made ­both ­using stan­dards and carot­id ­plaque.
Methods. Surgical spec­i­mens ­were ­obtained ­from ­patients under­go­ing carot­id endar­te­rec­to­my. Electrophoretic sep­ar­a­tions ­were per­formed a Waters Quanta 4000 instru­ment; for ­HPLC sep­ar­a­tion a Varian Vista 5500.
Results. The ­HPLC meth­od ­enables res­o­lu­tion in gra­di­ent of the ­four ­compounds in 30 min, ­while CE meth­od in 20 min.
Conclusions. The CE and ­HPLC sep­ar­a­tions of allan­toin, UA, Hx and X may be ­used for a sat­is­fac­to­ry deter­mi­na­tion in ­vivo, in ­short ­time. Moreover the CE sep­ar­a­tion ­gave ­more pre­cise allan­toin dos­age, ­even ­when the con­cen­tra­tion is low.

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