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A Journal on Applied Physiology, Biomechanics, Preventive Medicine,
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The Journal of Sports Medicine and Physical Fitness 2016 Nov 16

language: English

Reliability of salivary cortisol and Immunoglobulin-A measurements from the IPRO® before and after sprint cycling exercise

Luke A. MACDONALD 1, 2, Phillip M. BELLINGER 1, Clare L. MINAHAN 1

1 Griffith University Sports Science, School of Allied Health Sciences, Griffith University, Queensland, Australia; 2 Centre of Excellence for Applied Sport Science Research, Queensland Academy of Sport, Department of National Parks, Sport and Racing, Level 1 Queensland Sport & Athletics Centre, Nathan, Queensland, Australia


BACKGROUND: The purpose of this study was to evaluate the inter-day reliability of the IPRO method for determining resting and post-exercise salivary cortisol (sCort) and rate of salivary Immunoglobulin-A (sIgA) secretion.
METHODS: Fourteen males (32±11 years) performed two trials (T1 and T2) separated by 7 d, comprising saliva sampling before and 15 min after completion of two, 30-s Wingate Anaerobic Tests separated by 3.5 min (2 x WAnT). sCort increased after the 2 x WAnT in both trials (T1: p<0.001; T2: p<0.001), whereas rate of sIgA secretion decreased in both trials (T1: p=0.002; T2: p=0.002).
RESULTS: The intraclass correlation coefficients for resting and post-exercise sCort and rate of sIgA secretion ranged from 0.96-0.99. Mean reliability, expressed as the coefficient of variation (%) and the typical error of measurement over the two trials were resting sCort 9.4%, 0.14 ng·mL-1 (95% CL 0.1-0.2), post-exercise sCort 11.9%, 0.44 ng·mL-1 (95% CL 0.3- 0.7), resting rate of sIgA secretion 7.4%, 85.5 μg·mL-1·min-1 (95% CL 65.2-127.1) and post- exercise rate of sIgA secretion 10.5%, 82.9 μg·mL-1·min-1 (95% CL 63.2-123.1).
CONCLUSIONS: The IPRO is a reliable method for determining sCort and rate of sIgA secretion at rest and after sprint-cycling exercise.

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