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A Journal on Applied Physiology, Biomechanics, Preventive Medicine,
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The Journal of Sports Medicine and Physical Fitness 2015 Dec 23

language: English

Metabolomic profiles investigation on atheletes’ urine 35 minutes after an 800-meter race

Sun T., Wu Y., Wu X., Ma H.

Shanghai University of Sport, Shanghai, China


AIM: The main energy delivery system of 800 meters run is the glycolytic system. Running the 800 meters causes significant changes in our body, yet our understanding of how these changes occur is limited.
METHODS: We used an NMR-based metabolomics analysis to evaluate the metabolite profile changes in 19 young male athletes’ urine samples after 800-meter runs and provide an overall picture of its impact. Various multivariate data analysis methods, including principal component analysis (PCA), partial least squares-discrimination analysis (PLS-DA), and orthogonal projection of latent-structure-discrimination analysis (OPLS-DA) were applied to analyze the NMR data and thus identify possible correlations between the metabolite profile changes and the alterations in biological pathways.
RESULTS: The potential biological mechanism of an 800-meter race was finally elucidated based on the multivariate statistical analysis results. The levels of Lac, 2HIV, Leu, 2HIB, Ala, NAG, Pyr, Crn,FMA, Ino and Hyx were up-regulated in the post samples, whereas the levels of certain metabolites, including 3HIV, Cit, Tau, Gly and FOR were down-regulated in the post samples.
CONCLUSION: 1Our study provides novel insights into the 800-meter race metabolic characteristic. Separation of pre-from post-exercise samples was related to the Krebs cycle, Cori cycle, Cahill cycle, HIFs and ROS. 2Besides the Lac change, the increased concentrations of Ino, 2HIV concentrations in the post-exercise urine samples represent potential indices which indicate the high percent of glycolysis during the 800-meter run. 3The increase of concentrations of Hyx, 2HB may indicated oxidative stress with concomitant ROS generation in the athletes’ bodies during the 800-meter race.

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