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THE JOURNAL OF SPORTS MEDICINE AND PHYSICAL FITNESS
A Journal on Applied Physiology, Biomechanics, Preventive Medicine,
Sports Medicine and Traumatology, Sports Psychology
Indexed/Abstracted in: Chemical Abstracts, CINAHL, Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 1,111
Original articles OTHER AREAS
(Biochemistry, Immunology, Kinanthropometry, Neurology, Neurophysiology, Ophtalmology, Pharmacology, Phlebology, etc.)
The Journal of Sports Medicine and Physical Fitness 2010 March;50(1):85-92
Validating the salivary testosterone and cortisol concentration measures in response to short high-intensity exercise
Crewther B. T. 1,2, Lowe T. E. 3, Ingram J. 1, Weatherby R. P. 2 ✉
1 Health and Food Group, Plant and Food Research, Hamilton, New Zealand;
2 School of Exercise Science and Sport Management, Southern Cross University, Lismore, Australia;
3 Bay of Plenty Polytechnic, School of Applied Sciences, Tauranga, New Zealand
AIM: To validate the testosterone (T) and cortisol (C) concentration measures in saliva in response to short high-intensity exercise.
METHODS: Nine healthy males provided matching saliva and plasma samples before and after a 30-second Wingate cycle test. Saliva was assayed for T (Sal-T) and C (Sal-C) concentrations, and plasma for total T and total C, sex hormone-binding globulin, corticosteroid-binding globulin (CBG) and albumin concentrations. The plasma free and bioavailable hormones were calculated.
RESULTS: The Sal-T and plasma T correlations were weak to moderate (r=0.57-0.61) when examined between individuals (pooled data for all participants), but these relationships improved (r = 0.71-0.73) within individuals (data for each participant on average). The Sal-C and plasma C correlations were strong both between individuals (r=0.81-0.84) and within individuals (r=0.83-0.84). The peak relative increases in Sal-T (35±9%) and Sal-C (63±29%) concentrations exceeded the plasma total and/or free hormones, but not the bioavailable hormones. Albumin (10±3%) and CBG (16±4%) also increased with exercise, along with blood lactate (943±119%).
CONCLUSION: The Sal-T and Sal-C concentration measures were validated in response to short high-intensity exercise, especially for individuals. The hormonal changes in saliva were also more sensitive to exercise (i.e. greater relative responses) than the plasma total and/or free hormones, potentially arising from changes in the binding proteins and blood lactate. These findings support the use of saliva as a medium for steroid determination in sport.