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The Journal of Sports Medicine and Physical Fitness 2007 December;47(4):483-90

language: English

Short duration exhaustive aerobic exercise induces oxidative stress: a novel play-oriented volitional fatigue test

Kyparos A. 1, Salonikidis K. 2, Nikolaidis M. G. 1, 3, Kouretas D.

1 Department of Biochemistry and Biotechnology University of Thessaly, Larissa, Greece
2 Department of Physical Education and Sports Science Aristotle University of Thessaloniki, Serres, Greece
3 Department of Physical Education and Sports Science University of Thessaly, Trikala, Greece


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Aim. Exercise is associated with the generation of reactive oxygen and nitrogen species. This study examined the oxidative stress in response to a novel volitional fatigue test.
Methods. Eleven male college students performed a volitional fatigue test consisting of shuttle runs with a tennis racquet in the hand towards the left and right sidelines within the tennis singles court in an attempt to hit tennis balls until exhaustion. A tennis ball serving machine was adjusted to alternate feeds to the forehand and backhand sides of the subjects, standing at the baseline, at a frequency of 20 balls per minute.
Results. Mean time to volitional fatigue was 5.9±1.3 min and mean heart rate at volitional fatigue was 189±8.1 beats·min-1. The volitional fatigue test resulted in significant increases in blood thiobarbituric acid-reactive substances (22%), protein carbonyls (58%), catalase activity (143%), total antioxidant capacity (34%) and oxidized glutathione (GSSG, 81%) concentration, as well as significant decreases in reduced glutathione (GSH, 15%) concentration and GSH/GSSG ratio (56%) immediately postexercise, as compared to the pre-exercise concentration.
Conclusion. The data provide evidence that acute short duration exhaustive aerobic exercise in the form of a novel volitional fatigue test is capable of inducing oxidative stress. This novel test could serve as an alternative exercise modality to study oxidative stress.

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