Advanced Search

Home > Journals > The Journal of Sports Medicine and Physical Fitness > Past Issues > The Journal of Sports Medicine and Physical Fitness 1999 June;39(2) > The Journal of Sports Medicine and Physical Fitness 1999 June;39(2):123-7



A Journal on Applied Physiology, Biomechanics, Preventive Medicine,
Sports Medicine and Traumatology, Sports Psychology

Indexed/Abstracted in: Chemical Abstracts, CINAHL, Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 1,111

Frequency: Monthly

ISSN 0022-4707

Online ISSN 1827-1928


The Journal of Sports Medicine and Physical Fitness 1999 June;39(2):123-7


Can oxy­pu­rines plas­ma lev­els clas­si­fy the ­type of phys­i­cal exer­cise?

Bianchi G. P. 1, Grossi G. 2, Bargossi A. M. 2, Fiorella P. L. 2, Marchesini G. 1

1 Division of Internal Medicine, Cardiology, Hepathology, University of Bologna, Italy;
2 Centralized Laboratory, Policlinico S. Orsola, Bologna, Italy

Background. A labor­a­to­ry-­based mod­el ­able to ­describe mus­cle ener­gy stat­us dur­ing phys­i­cal exer­cise and chang­es in myo­fi­bril­lar com­po­si­tion in ­response to train­ing ­would be desir­able. Lactate and ammo­nia con­cen­tra­tions are not suf­fi­cient for a com­pre­hen­sive knowl­edge of ­these ­systems. All mus­cle ­fibres, irre­spec­tive of the ­type, ­show ATP deple­tion and IMP accu­mu­la­tion fol­low­ing exhaust­ing mus­cu­lar exer­cise ­with quan­ti­ta­tive dif­fer­enc­es due to the dif­fer­ent con­cen­tra­tions of deam­i­nase. We stud­ied the plas­ma con­cen­tra­tion of metab­olites of oxy­pu­rine cas­cade to ­test ­their reli­abil­ity to clas­si­fy dif­fer­ent exer­cis­es.
Methods. We stud­ied 52 ath­letes, meas­ur­ing plas­ma metab­olites at the begin­ning and at the end of ­their spec­i­fic ­field exer­cise (cycle pur­suers, 8 cas­es; soc­cer ­players, 19; mar­a­thon run­ners, 25). K3­EDTA-­blood sam­ples ­were ­assayed for plas­ma hypox­an­thine, xan­thine, and ­inosine, ­using an ­HPLC tech­nique, as ­well as ammo­nia and lac­tate by ­means of enzy­mat­ic meth­ods.
Results and con­clu­sions. Basal oxy­pu­rines lev­els ­were not dif­fer­ent in rela­tion to any spe­cif­ic phys­i­cal exer­cise. Post-exer­cise oxy­pu­rines, name­ly hypox­an­thine, ­were ­more pre­cise pre­dic­tors of mus­cle ener­gy exhaus­tion ­than ­strain inten­sity or dura­tion. Plasma lev­els of hypox­an­thine may be ele­vat­ed ­also in the pres­ence of nor­mal xan­thine and ­uric ­acid con­cen­tra­tions, due to an exhaus­tion of the enzy­mat­ic path­way, to a ­reduced activ­ity of xan­thine-oxi­dase or final­ly to a sub­strate-depen­dent inhi­bi­tion of the pro­cess.

language: English


top of page