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A Journal on Applied Physiology, Biomechanics, Preventive Medicine,
Sports Medicine and Traumatology, Sports Psychology

Indexed/Abstracted in: Chemical Abstracts, CINAHL, Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 1,111

Frequency: Monthly

ISSN 0022-4707

Online ISSN 1827-1928


The Journal of Sports Medicine and Physical Fitness 1998 June;38(2):111-5


Effects of inten­sive endu­rance exer­cise on DNA dam­age in leu­coc­y­tes

Niess A. M., Baumann M., Roecker K., Horstmann T., Mayer F., Dickhuth H. H.

Medical Clinic and Polyclinic, Division of Sport Medicine, University of Tübingen, Ger­ma­ny

Objec­tive. It has ­been ­shown ­that high­ly inten­sive anaer­o­bic exer­cise induc­es DNA dam­age in leu­coc­y­tes (LEU). The ­present ­study was ­designed to inves­ti­gate wheth­er inten­sive endu­rance exer­cise is ­capable of induc­ing com­par­able ­effects.
Experi­men­tal ­design. A pros­pec­tive ­study.
Par­tic­i­pants. ­Twelve men (­aged 27.3±4.1 ­years) who under­took a reg­u­lar train­ing of dif­fer­ent ­extent (run­ning vol­ume 45±25 km· ­week-1) vol­un­teered in the ­study.
Inter­ven­tions. The sub­jects com­pet­ed in a ­half mar­a­thon (HM) of 21.1 km, 93.0±10.4 min.
Meas­ure­ments. ­Blood was tak­en at ­rest, 1 and 24 hrs ­after HM for deter­mi­na­tion of crea­tine ­kinase, neu­troph­il (PMN), lym­pho­cyte and mono­cyte ­counts. DNA dam­age in LEU at ­rest and 24 hrs ­after HM was quan­ti­fied ­using the sin­gle ­cell gel-elec­tro­pho­re­sis (SCG) ­assay.
­Results. PMN ­increased ­from 2.81±0.69 to 13.13±2.91 1 hrs ­after HM (p<0.01) and ­returned to 3.26±0.47 109 ­cells·l-1 by 24 hr recov­ery. DNA migra­tion (­image ­length, IL) reflect­ing the ­extent of DNA dam­age was ele­vat­ed sig­nif­i­cant­ly in 10 of 12 sub­jects one day ­after HM. IL ­rose ­from 32.7±2.2 to 40.7±3.9 µm (p<0.01). Cor­re­la­tion anal­y­sis ­revealed a rela­tion­ship ­between DNA migra­tion 24 hrs ­after HM and PMN ­count 1 hr ­post-exer­cise (r=0.67, p<0.05).
Con­clu­sions. The ­results con­firm the hypoth­e­sis ­that DNA dam­age in LEU ­occurs ­after inten­sive endu­rance exer­cise. We sup­pose our obser­va­tion of exer­cise-­induced DNA dam­age in LEU is affect­ed by reac­tive oxy­gen spe­cies ­which are ­released ­from PMN. It is ­quite ­unclear wheth­er DNA dam­age in LEU is cau­sal ­involved in exer­cise-­induced mod­ifi­ca­tions of the ­immune ­system.

language: English


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