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A Journal on Internal Medicine


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ORIGINAL ARTICLES  


Panminerva Medica 2015 December;57(4):177-82

language: English

Pharmacokinetic study of isatin in dog plasma by liquid chromatography tandem mass spectrometry

Ren A. 1, Wang Q. 1, Fang Z. 1, Gao M. 1, Wang H. 3, Zhang J. 1, Xu W. 2, Yue W. 2, Yin L. 2, Liu Z. 2, Li X. 2, Ding B. 1

1 Shandong Provincial Hospital affiliated to Shandong University, Jinan, China;
2 The affiliated Hospital of Qingdao University, Qingdao, China;
3 The Hospital of Luzhong Mining Co., Ltd. , Laiwu, China


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AIM: A sensitive and selective method was developed and validated to study the pharmacokinetics of isatin.
METHODS: The blood samples were pretreated by protein precipitation method using methanol. Quetiapine was used as an internal standard. After pretreatment, the samples were assayed by LC/MS/MS method and the pharmacokinetic parameters were calculated by WinNonlin 5.2 using non-compartment model. The separation was performed on a Venusil XBP PH column (5 µm, 2.0×100 mm) with an isocratic mobile phase consisted of methanol-water (containing 50 mM ammonium formate) (65:35, v/v) at a flow rate of 0.3 mL/min. The Agilent G6410B triple quadrupole LC/MS system was operated under the multiple reactions monitoring mode (MRM) using the electrospray ionization technique in positive mode.
RESULTS: The lower limits of quantification (LLOQ) of the analyte of the method was 10 ng/mL. The method was linear with correlation coefficient >0.995. The intraday and interday accuracy and precision of the assay were acceptable.
CONCLUSION: This method has been applied successfully to a pharmacokinetic study involving the oral and intravenous administration of isatin to beagle dogs.

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