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A Journal on Internal Medicine

Indexed/Abstracted in: BIOSIS Previews, Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
Impact Factor 1,6

Frequency: Quarterly

ISSN 0031-0808

Online ISSN 1827-1898


Panminerva Medica 2003 March;45(1):63-7


Nucleolar Organizer Regions (NORs). Their significance in the determination of the origin of the lymphoid vessels

Papadopoulos N. 1, Simopoulos C. 2, Hatzimichael A. 3, Kotini A. 4, Tamiolakis D. 5

1 Department of Histology-Embryology Democritus University of Thrace, Greece
2 Department of Experimental Surgery
3 Department of Pediatrics
4 Department of Medical Physics
5 Department of Cytology Regional Hospital of Alexandroupolis, Greece

Aim. The aim of ­this ­study is the eval­u­a­tion of the cel­lular ­activity of the ­embryonal mesen­chyme in ­order to inves­ti­gate if the prim­i­tive ­lymph ves­sels are ­derived ­from ­clefts of the mesen­chyme or ­from cap­il­lary off­shoots ­from the endo­the­lium of the ­veins. For ­this ­reason we ­applied the quan­ti­ta­tive esti­ma­tion of nucle­olar orga­nizer ­regions (NORs) in the endo­the­lial ­cells of the ­lymph and ­blood ves­sels, as ­well as in the mesen­chymal ­cells ­that sur­round ­these ves­sels.
Methods. Our mate­rial con­sisted of the his­to­log­ical sec­tions ­from the ­proper mesen­te­rium of ­30 ­embryos in dif­ferent ­stages of devel­op­ment (10th, 12th, 15th, 20th, 23th and 30th ­week).
Results. A sta­tis­ti­cally sig­nif­i­cant dif­fer­ence in the ­average AgNORs ­value in the endo­the­lial (­ANOVA, p<0.0001) and the mesen­chymal ­cells (­ANOVA, p<0.0001) ­between ­lymph ves­sels, ­blood ves­sels at ­close and ­blood ves­sels at dis­tant ­regions (10th, 12th, 15th ­week) was ­found. There was no sta­tis­ti­cally sig­nif­i­cant dif­fer­ence of the ­average AgNORs ­value in the endo­the­lial (­ANOVA, p=0.55) and the mesen­chymal ­cells (­ANOVA, p=0.49) ­between ­lymph ves­sels, ­blood ves­sels at ­close and ­blood ves­sels at dis­tant ­regions (20th, 23th, 30th ­week).
Conclusion. The ­above ­results sug­gest ­that the devel­op­ment of the ­lymph ves­sels fol­lows ­that of the ­blood ves­sels. Furthermore, the ­intense pro­tein syn­thesis ­that is ­expressed by ­means of NORs in the mesen­chymal ­cells sur­rounding ­lymph ves­sels ­from the 10th to the 15th ­week of devel­op­ment is an addi­tional ­proof of the ­view ­that the prim­i­tive ­lymph ves­sels are ­derived ­from ­clefts ­into the ­embryonal mesen­chyma and not ­from cap­il­lary off­shoots of the endo­the­lium of the ­blood ves­sels.

language: English


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