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Panminerva Medica 2003 March;45(1):63-7

language: English

Nucleolar Organizer Regions (NORs). Their significance in the determination of the origin of the lymphoid vessels

Papadopoulos N. 1, Simopoulos C. 2, Hatzimichael A. 3, Kotini A. 4, Tamiolakis D. 5

1 Department of Histology-Embryology Democritus University of Thrace, Greece
2 Department of Experimental Surgery
3 Department of Pediatrics
4 Department of Medical Physics
5 Department of Cytology Regional Hospital of Alexandroupolis, Greece


Aim. The aim of ­this ­study is the eval­u­a­tion of the cel­lular ­activity of the ­embryonal mesen­chyme in ­order to inves­ti­gate if the prim­i­tive ­lymph ves­sels are ­derived ­from ­clefts of the mesen­chyme or ­from cap­il­lary off­shoots ­from the endo­the­lium of the ­veins. For ­this ­reason we ­applied the quan­ti­ta­tive esti­ma­tion of nucle­olar orga­nizer ­regions (NORs) in the endo­the­lial ­cells of the ­lymph and ­blood ves­sels, as ­well as in the mesen­chymal ­cells ­that sur­round ­these ves­sels.
Methods. Our mate­rial con­sisted of the his­to­log­ical sec­tions ­from the ­proper mesen­te­rium of ­30 ­embryos in dif­ferent ­stages of devel­op­ment (10th, 12th, 15th, 20th, 23th and 30th ­week).
Results. A sta­tis­ti­cally sig­nif­i­cant dif­fer­ence in the ­average AgNORs ­value in the endo­the­lial (­ANOVA, p<0.0001) and the mesen­chymal ­cells (­ANOVA, p<0.0001) ­between ­lymph ves­sels, ­blood ves­sels at ­close and ­blood ves­sels at dis­tant ­regions (10th, 12th, 15th ­week) was ­found. There was no sta­tis­ti­cally sig­nif­i­cant dif­fer­ence of the ­average AgNORs ­value in the endo­the­lial (­ANOVA, p=0.55) and the mesen­chymal ­cells (­ANOVA, p=0.49) ­between ­lymph ves­sels, ­blood ves­sels at ­close and ­blood ves­sels at dis­tant ­regions (20th, 23th, 30th ­week).
Conclusion. The ­above ­results sug­gest ­that the devel­op­ment of the ­lymph ves­sels fol­lows ­that of the ­blood ves­sels. Furthermore, the ­intense pro­tein syn­thesis ­that is ­expressed by ­means of NORs in the mesen­chymal ­cells sur­rounding ­lymph ves­sels ­from the 10th to the 15th ­week of devel­op­ment is an addi­tional ­proof of the ­view ­that the prim­i­tive ­lymph ves­sels are ­derived ­from ­clefts ­into the ­embryonal mesen­chyma and not ­from cap­il­lary off­shoots of the endo­the­lium of the ­blood ves­sels.

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