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A Journal on Internal Medicine

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Panminerva Medica 2002 December;44(4):365-7

language: English

CD 34 expression in chronic and neoplastic liver diseases

Di Carlo I. 1, Fraggetta F. 2, Lombardo R. 1, Azzarello G. 1, Vasquez E. 2, Puleo S. 1

1 Department of Surgical Sciences, Organ Transplantation and Advanced Technologies, University of Catania, Catania, Italy
2 Institute of Pathologic Anatomy, University of Catania, Catania, Italy


Background. Capillarisation of hepat­ic sin­u­soids is a ­well rec­og­nized phe­no­men occur­ring in ­long stand­ing liv­er dis­ease, in hepat­ic cir­rho­sis as ­well as in hepat­o­cel­lu­lar car­ci­no­ma. To ­study immu­no­his­to­chem­i­cal­ly the expres­sion and dis­tri­bu­tion of CD34 in chron­ic liv­er dis­ease and hepat­o­cel­lu­lar car­ci­no­ma in ­order to eval­u­ate the pos­sible diag­nos­tic impli­ca­tion of ­this mark­er.
Methods. Sixty-­five sam­ples of liv­er tis­sue show­ing nor­mal liv­er, dif­fer­ent ­degrees of chron­ic inflam­ma­tion, cir­rho­sis and his­to­log­i­cal fea­tures of hepat­o­cel­lu­lar aden­o­ma and car­ci­no­ma (HCC) ­were includ­ed in the ­study. The spec­i­mens ­were ­fixed in for­mal­in and embed­ded in par­af­fin and an immu­no­his­to­chem­i­cal inves­ti­ga­tion was per­formed by the stan­dard avi­din-bio­tin-per­ox­i­dase com­plex meth­od ­with CD34.
Results. The sin­u­soids of nor­mal liv­er ­showed no immu­no­reac­tiv­ity. The sin­u­soids of liv­er affect­ed by dif­fer­ent ­degrees of chron­ic ­active hep­a­titis ­showed no or ­focal immu­nos­tain­ing for CD34; an ­increased immu­no­reac­tiv­ity was ­observed in the per­i­por­tal sin­u­soids of the cir­rhot­ic nod­ules where­as dif­fuse and ­strong stain­ing was ­observed in the over­all HCC as ­well as in the hepat­o­cel­lu­lar aden­o­ma test­ed.
Conclusions. In HCC, immu­no­reac­tiv­ity for CD34 rep­re­sents an effec­tive meth­od to eval­u­ate angio­gen­e­sis and to dis­tin­guish ­well-dif­fer­en­tiat­ed HCC ­from non-neo­plas­tic liv­er. Its ­role in clin­i­cal ­stage and prog­nos­tic eval­u­a­tion ­needs fur­ther inves­ti­ga­tion.

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