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A Journal on Internal Medicine

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Panminerva Medica 1999 December;41(4):319-22

language: English

Cytotoxic effec­tor func­tion in HLA-asso­ciat­ed auto­im­mune dis­eas­es

Pugli­si F., Cap­u­a­no P., Ver­zil­lo F., Simone M., Lau­rent­a­ci C., Catalano G.

From the Chair of General Surgery I Faculty of Medicine and Surgery University of Bari, Bari, Italy


Background. The mech­a­nisms by ­which cyto­tox­ic-T-lym­pho­cytes (CTLs) and nat­u­ral kill­er (NK) ­cells rec­og­nize and ­kill tar­get ­cells are the sub­ject of ­intense ­research. Previous obser­va­tions in ­patients ­with HLA-asso­ciat­ed auto­im­mune dis­eas­es ­show a ­major ­impact of ­cell-medi­at­ed immu­nity as com­pared to anti­body-medi­at­ed immu­nity. This ­study ana­lyz­es the pos­sible co-oper­a­tion of NK ­cells in the auto­im­mune pro­cess, com­par­ing ­their ­increase to ­that of cyto­tox­ic-T-lym­pho­cytes.
Methods. This reas­earch exam­ines the inci­dence of CD8+ T ­cells and NK ­cells increas­es in 1065 ­patients ­with var­i­ous auto­im­mune dis­eas­es. Tissue typ­ing for HLA anti­gens was car­ried out in all ­patients by con­ven­tion­al ser­o­log­ic meth­ods. Lymphocyte sub­sets (CD4+, CD8+, CD3-HLA-DR+, NK ­cells, sIg+ B ­cells) ­were detect­ed ­with mono­clo­nal anti­bod­ies by a flu­o­res­cent cytom­e­ter. Results ­were sta­tis­ti­cal­ly com­pared to nor­mal con­trol val­ues by the Student’s “t”-­test.
Results. The anal­y­sis of ­results ­shows the inci­dence of CTLs and NK ­cell increas­es in ­patients ­with dif­fer­ent auto­im­mune syn­dromes. In ­some dis­eas­es (­uveitis, mul­ti­ple scler­o­sis, and oth­er neu­ro­log­i­cal auto­im­mune dis­or­ders) ­raised fre­quen­cies of NK ­cell ­increase are ­most evi­dent. These ­results sug­gest ­some pos­sible rela­tion­ships ­between CTLs and NK ­cells in the path­o­gen­e­sis of the auto­im­mune pro­cess.
Conclusions. The ­present ­results ­show ­that CTLs and NK ­cells are impli­cat­ed in ­immune dys­reg­u­la­tion, ­which char­ac­teriz­es the com­plex path­o­gen­e­sis of auto­im­mune dis­eas­es.

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