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A Journal on Otorhinolaryngology, Head and Neck Surgery,
Plastic Reconstructive Surgery, Otoneurosurgery

Indexed/Abstracted in: EMBASE, Scopus




Otorinolaringologia 2011 December;61(4):127-41

language: English

Bcl-2 prevents hearing loss in a mouse model of sound trauma

Pfannenstiel S. C. 1, 2, Praetorius M. 1, 2, Imig T. J. 3, Plinkert P.-K. 2, Brough D. E. 4, Staecker H. 1

1 Department of Otolaryngology Head and Neck Surgery, University of Kansas School of Medicine, Kansas City, KS, USA
2 Department of Otolaryngology Head and Neck Surgery, University of Heidelberg, Heidelberg, Germany
3 Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA
4 GenVec Inc., Gaithersburg, MA, USA


Over-expression of bcl-2 has been demonstrated to prevent hair cell death in an in vitro and in an in vivo ototoxicity model. To determine if the mechanism of injury influences the protective effects of bcl-2 we have developed a mouse model of bcl-2 delivery after sound trauma. Adult C57Bl/6 mice underwent a sound exposure of 115 dB at 16 kHz for two hours. Auditory brain stem response and distortion product otoacoustic emissions were evaluated prior sound trauma and at 24 days after sound trauma. Finally, the cochleae were removed and the inner ear examined by thick serial sections under confocal microscopy to establish the degree of hair cell loss. A second group of mice was pretreated with an advanced generation adenovector expressing human bcl-2 delivered via the posterior semicircular canal. Seventy-two hours after vector delivery mice were exposed to sound. Hearing thresholds were evaluated prior to vector delivery, sound exposure and at 10 and 24 days post-sound exposure. Subsequently hair cell counts were determined. Bcl-2-pretreatment was found to protect hair cells and preserve hearing following sound trauma compared to control-vector-pretreated mice and sound-only treated mice.

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