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A Journal on Neurosurgery

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Journal of Neurosurgical Sciences 2015 May 12


language: English

Induced bone marrow stromal cells into Schwann cells by progesterone improved the outcome of transected sciatic nerve model

Movaghar B. 1, Tiraihi T. 1, 2, Javan M. 3, Taheri T. 2, Kazemi H. 2

1 Department of Anatomical Sciences, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran; 2 Shefa Neyrosciences Research Center, Khatam Al-Anbia Hospital, Tehran, Iran; 3 Department of Physiology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran


Peripheral nerve injury is a common lesion in clinical practice and transplantation is one of the approaches to its treatment. While nerve graft is used for restoring the defected nerve using autologous or allogenic tissues, Schwann cells are considered as an alternative source. In this investigation, bone marrow stromal cells (BMSCs) were induced into Schwann-­like cells (SLCs) using progesterone. The BMSCs were collected from the long bones of rats and were in vitro transdifferentiated into SLCs by preinduction with β-­mercaptoethanol and retinoic acid, which was followed by induction with bFGF, PDGF, forskelin and progesterone. The SLCs were transplanted in a rat model of the sciatic nerve injury with 1 cm gaps. A sciatic function index (SFI), histological, immunohistochemical and ultrastructural studies were used in evaluating the improvement in the nerves regeneration. The results show significant differences in the SFI between the control and the treated groups (P<0.05). The transplant was immunoreactive to S100, and the electron microscopy showed myelination in the transplanted cells. The conclusion of the study is that there were functional and structural improvements in the progesterone induced SLCs, which were not significantly different from the heregulin treated ones (positive control) but significantly so from negative controls.

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