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Journal of Neurosurgical Sciences 2001 March;45(1):1-6

Copyright © 2001 EDIZIONI MINERVA MEDICA

language: English

Inverse correlation of TRF1 expression and cell proliferation in human primary intracranial tumors

De Divitiis O., La Torre D.

Department of Neurosurgery, Polyclinic, University of Messina, Messina, Italy



Background. The tel­o­mer­ic-­repeat bind­ing fac­tor (TRF1) par­tic­i­pates in a phys­io­log­i­cal homeo­stat­ic mech­a­nism con­trol­ling cel­lu­lar pro­life­ra­tive poten­tial. TRF1 is ­involved in a neg­a­tive feed­back mech­a­nism ­that ­allows tel­o­mere short­en­ing by inhib­it­ing the activ­ity of telom­e­rase. Down-reg­u­la­tion of TRF1 expres­sion ­results in tel­o­mere elon­ga­tion and may be ­involved in ­cell immor­tal­iza­tion. The ­goal of the ­present ­study was to deter­mine wheth­er rou­tine immu­no­his­to­chem­i­cal (IHC) tech­niques can char­ac­ter­ize TRF1 expres­sion in dif­fer­ent ­human ­brain ­tumor spec­i­mens and wheth­er it cor­re­lates ­with oth­er indi­ces of ­brain ­tumor’s pro­life­ra­tive poten­tial.
Methods. A ­cohort of 20 ­flash-fro­zen sur­gi­cal spec­i­mens [14 menin­gio­mas and 6 ana­plas­tic astro­cy­to­mas (AA)] ­were eval­u­at­ed for TRF1 expres­sion. Results of par­allel inves­ti­ga­tions of ­tumor’s pro­life­ra­tive indi­ces as ­assessed by Ki67 label­ing ­index (LI) deter­mi­na­tions ­were ­cross-cor­re­lat­ed ­with TRF1 expres­sion ­results and his­to­type.
Results. We dem­on­strat­ed var­i­able lev­els of TRF1 expres­sion in 12 out of 14 (87.5%) menin­gio­ma sam­ples. By con­trast, we detect­ed no expres­sion of TRF1 in tis­sue sam­ples ­from AA (p=0.008). The Ki67 LI was high­er in AA ­than in menin­gio­ma sam­ples (15.21±9.34 vs 26.6±13.89, p=0.044). Statistical anal­y­sis ­revealed a sig­nif­i­cant ­inverse cor­re­la­tion ­between TRF1 expres­sion, his­to­type, and LI (χ2=14.1; p=0.0008).
Conclusions. We dem­on­strat­ed for the ­first ­time ­that rou­tine IHC tech­niques are ­capable to iden­ti­fy­ing TRF1 expres­sion in intra­cra­ni­al ­tumors. The ­results sug­gest ­that TRF1 is het­er­o­ge­ne­ous­ly ­expressed in menin­gio­mas, and ­absent in AA. The TRF1 stat­us in intra­cra­ni­al ­tumors ­might be of prog­nos­tic val­ue and pos­sibly rep­re­sent a poten­tial appli­ca­tion for bio­log­i­cal­ly tar­get­ed ther­a­peu­tic strat­e­gies.

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