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Home > Journals > Minerva Stomatologica > Past Issues > Minerva Stomatologica 2013 July-August;62(7-8) > Minerva Stomatologica 2013 July-August;62(7-8):267-80



A Journal on Dentistry and Maxillofacial Surgery

Official Journal of the Italian Society of Odontostomatology and Maxillofacial Surgery
Indexed/Abstracted in: CAB, EMBASE, Index to Dental Literature, PubMed/MEDLINE, Scopus, Emerging Sources Citation Index

Frequency: Bi-Monthly

ISSN 0926-4970

Online ISSN 1827-174X


Minerva Stomatologica 2013 July-August;62(7-8):267-80


Effects of titanium surface topography on morphology and in vitro activity of human gingival fibroblasts

Ramaglia L. 1, Capece G. 1, Di Spigna G. 2, Bruno M. P. 1, Buonocore N. 1, Postiglione L. 2

1 Department of Oral and Maxillo-Facial Sciences Federico II University, Naples, Italy;
2 Department of Cellular and Molecular Biology and Pathology Federico II University, Naples, Italy

Aim: The aim of the present study was to evaluate in vitro the biological behavior of human gingival fibroblasts cultured on two different titanium surfaces.
Methods: Titanium test disks were prepared with a machined, relatively smooth (S) surface or a rough surface (O) obtained by a double acid etching procedure. Primary cultures of human gingival fibroblasts were plated on the experimental titanium disks and cultured up to 14 days. Titanium disk surfaces were analysed by scanning electron microscopy (SEM). Cell proliferation and a quantitative analysis by ELISA in situ of ECM components as CoI, FN and TN were performed.
Results: Results have shown different effects of titanium surface microtopography on cell expression and differentiation. At 96 hours of culture on experimental surfaces human gingival fibroblasts displayed a favourable cell attachment and proliferation on both surfaces although showing some differences.
Conclusion: Both the relatively smooth and the etched surfaces interacted actively with in vitro cultures of human gingival fibroblasts, promoting cell proliferation and differentiation. Results suggested that the microtopography of a double acid-etched rough surface may induce a greater Co I and FN production, thus conditioning in vivo the biological behaviour of human gingival fibroblasts during the process of peri-implant soft tissue healing.

language: English, Italian


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