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A Journal on Dentistry and Maxillofacial Surgery
Minerva Stomatologica 2013 June;62(6):199-205
Influence of different photo-activation distances on cytotoxicity of a dental adhesive model resin
De Carvalho R. V. 1, Fernández M. R. 2, Poli-Frederico R. C. 1, Guiraldo R. D. 1, Lopes M. B. 1, Berger S. B. 1, Moura S. K. 1, Demarco F. F. 3 ✉
1 Department of Restorative Dentistry, School of Dentistry, University of North Parana, Londrina, PR, Brazil;
2 Department of Restorative Dentistry, School of Dentistry Pierre Fauchard, Autonomous University of Paraguay, Asuncion, Paraguay;
3 Department of Operative Dentistry, School of Dentistry, Federal University of Pelotas, RS, Brazil
Aim: This study evaluated the cytotoxicity of a dental bonding model resin (DBMR) submitted to different photo-activation distances.
Methods: A monomer mixture based on Bis-GMA and HEMA was used to assess the cytotoxicity in a mouse fibroblast-cell line. To promote different photo-activation distances glass slides were interposed between DBMR surface and halogen light curing unit (LCU) tip. Afterwards, the specimens were immersed in RPMI culture medium for 24 h to obtain extracts. The extracts were incubated in contact with the cells for 24 h. Finally, an MTT colorimetric assay was used to assess the cytotoxicity. The cell viability data (absorbance) were analyzed by one way ANOVA followed by Tukey’s test (P<0.05).
Results: The light output decreased according to the increase in the number of glass slides between the halogen LCU tip and DBMR surface. Yet, the distance between the tip of the curing light system and the specimens had significant influence on the cytotoxicity. All extracts produced by groups submitted to different photo-activation distances showed cytotoxic effect after 24h of incubation.
Conclusion: The photo-activation distance and the interposition of glass slides between LCU tip and DBMR was shown to play an important role in the cytotoxic effect.